Detailed information of TA system TA196635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1136497..1137992
• Replicon: chromosome
• Accession: NZ_CP071632
• Organism: Rhizobium sp. NZLR1

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: J3O30_RS05620
• Protein ID: WP_207583278.1
• Coordinates: 1136497..1137765 (-)
• Length: 423 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: J3O30_RS05625
• Protein ID: WP_207583279.1
• Coordinates: 1137762..1137992 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3O30_RS05595	1132221..1133270	+	1050	WP_207583274.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
J3O30_RS05600	1133280..1133987	+	708	WP_207583275.1	hypothetical protein	-
J3O30_RS05605	1133976..1134261	-	286	Protein_1102	DUF2293 domain-containing protein	-
J3O30_RS05610	1134466..1135347	-	882	WP_207583276.1	GIY-YIG nuclease family protein	-
J3O30_RS05615	1135581..1136360	-	780	WP_207583277.1	SDR family NAD(P)-dependent oxidoreductase	-
J3O30_RS05620	1136497..1137765	-	1269	WP_207583278.1	HipA domain-containing protein	Toxin
J3O30_RS05625	1137762..1137992	-	231	WP_207583279.1	type II toxin-antitoxin system Y4mF family antitoxin	Antitoxin
J3O30_RS05630	1138416..1139021	-	606	WP_207583280.1	WHG domain-containing protein	-
J3O30_RS05635	1139133..1139819	+	687	WP_207583281.1	PspA/IM30 family protein	-
J3O30_RS05640	1139870..1140151	+	282	WP_003588051.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (151-385)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 423 a.a.
• Molecular weight: 45976.59 Da
• Isoelectric Point: 5.2504

>T196635 WP_207583278.1 NZ_CP071632:c1137765-1136497 [Rhizobium sp. NZLR1]
MTDRILNVWWNGRIVGQFTQDRHGDIGFAYAEGWLGDDDALPLSASLPKRPERFSRRECRPFFGGLLPEENQRIAAAQAL
GVSPANDFALLDHLGGDVAGALQLLAEGQEPAETATTADRQSAPLDEAGIVRVLEALPVRPLLAGEEGLRLSLAGAQSKV
PVVLIDGRIGLPLPGQATTHILKPPIARFKATTENEAFVMRLAAAIELDVAAVEPRHVRERPFLLVERYDRVRDAGGLVR
RIHQEDFCQALGVPPETKYASEGGPTFKDCFELLRRISARPAIDVLKLLDAAIFNLIAGNADAHGKNFSILYDSQGPRLA
KLYDLLSTVIYPELSPKLAMRIGKRATLAEMDAEGWQVFAREAGVGTPLLRRRVNELCDKVIAAADKVADELADISPDRD
PIDEIAGLIKSRAEAARLTSKD

Nucleotide

• Length: 1269 bp

>T196635 NZ_CP095236:14827-15138 [Klebsiella pneumoniae]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8196.57 Da
• Isoelectric Point: 10.5957

>AT196635 WP_207583279.1 NZ_CP071632:c1137992-1137762 [Rhizobium sp. NZLR1]
MSKQIKTPADIGALVRSTRKEQNLRQDELAGVAGVGLRFIVDLEAGKPTAQIGKVLQVLQTLGCSIDILAPGERRR

Nucleotide

• Length: 231 bp

>AT196635 NZ_CP095236:15135-15554 [Klebsiella pneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA