Detailed information of TA system TA196625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3404760..3405433
• Replicon: chromosome
• Accession: NZ_CP071626
• Organism: Rhizobium leguminosarum strain GLR17

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J3P71_RS17075
• Protein ID: WP_020050800.1
• Coordinates: 3404760..3405173 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J3P71_RS17080
• Protein ID: WP_020050801.1
• Coordinates: 3405170..3405433 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3P71_RS17065	3402162..3403370	-	1209	WP_168306498.1	efflux RND transporter periplasmic adaptor subunit	-
J3P71_RS17070	3403639..3404580	-	942	WP_168306499.1	adenylate/guanylate cyclase domain-containing protein	-
J3P71_RS17075	3404760..3405173	-	414	WP_020050800.1	type II toxin-antitoxin system VapC family toxin	Toxin
J3P71_RS17080	3405170..3405433	-	264	WP_020050801.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J3P71_RS17085	3405522..3407072	-	1551	WP_168306500.1	SpoVR family protein	-
J3P71_RS17090	3407069..3408343	-	1275	WP_020050803.1	YeaH/YhbH family protein	-
J3P71_RS17095	3408356..3410299	-	1944	WP_020050804.1	PrkA family serine protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (9-54)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15311.73 Da
• Isoelectric Point: 5.6789

>T196625 WP_020050800.1 NZ_CP071626:c3405173-3404760 [Rhizobium leguminosarum]
MTAWMLDTNMVSHVIKGDRPEIIDRLAALPMEDIVISSITEGELFYGLAKRGYPKVLSERIRQFLLRVDVLPWDHDVTRT
YGDLRAACEAKGVTLAPLDMMIAAHAVATAATLVTRDKAFARVSEPLKVDDWANERS

Nucleotide

• Length: 414 bp

>T196625 NZ_CP095234:c1792256-1791972 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10153.26 Da
• Isoelectric Point: 5.1859

>AT196625 WP_020050801.1 NZ_CP071626:c3405433-3405170 [Rhizobium leguminosarum]
MATRTAKIFTTGRSQAVRLPAEFRFEESEVFVRRDPKTGDVILSRKPDSWDGLFELYGKDRVPDDFLGPDDRHQPSHDRD
PFEGWKE

Nucleotide

• Length: 264 bp

>AT196625 NZ_CP095234:c1792488-1792246 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA