Detailed information of TA system TA196623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2420335..2420921
• Replicon: chromosome
• Accession: NZ_CP071626
• Organism: Rhizobium leguminosarum strain GLR17

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A6N9ZKV3
• Locus tag: J3P71_RS12235
• Protein ID: WP_072638505.1
• Coordinates: 2420661..2420921 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J3P71_RS12230
• Protein ID: WP_204347447.1
• Coordinates: 2420335..2420664 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3P71_RS12200	2415441..2416067	+	627	WP_168302989.1	isoprenylcysteine carboxylmethyltransferase family protein	-
J3P71_RS12205	2416361..2416570	+	210	WP_131642634.1	hypothetical protein	-
J3P71_RS12210	2416580..2416954	+	375	WP_168302990.1	GFA family protein	-
J3P71_RS12215	2417116..2418408	+	1293	WP_168302991.1	efflux RND transporter periplasmic adaptor subunit	-
J3P71_RS12220	2418421..2419137	+	717	WP_168302992.1	ABC transporter ATP-binding protein	-
J3P71_RS12225	2419127..2420329	+	1203	WP_168302993.1	ABC transporter permease	-
J3P71_RS12230	2420335..2420664	-	330	WP_204347447.1	toxin-antitoxin system HicB family antitoxin	Antitoxin
J3P71_RS12235	2420661..2420921	-	261	WP_072638505.1	type II toxin-antitoxin system HicA family toxin	Toxin
J3P71_RS12240	2421091..2421450	+	360	WP_168302994.1	VOC family protein	-
J3P71_RS12245	2421608..2422222	+	615	WP_168302995.1	GntR family transcriptional regulator	-
J3P71_RS12250	2422273..2423085	-	813	WP_168302996.1	ferredoxin--NADP reductase	-
J3P71_RS12255	2423442..2424170	+	729	WP_012757415.1	phosphatidylcholine synthase	-
J3P71_RS12260	2424181..2425161	+	981	WP_168302997.1	quinone oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (25-83)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9509.85 Da
• Isoelectric Point: 10.9269

>T196623 WP_072638505.1 NZ_CP071626:c2420921-2420661 [Rhizobium leguminosarum]
MTVSGKHRTTLEAIFADPVRASIAWRDIEALFVACGGEVSEGNGSRVRVALNGVRAVFHRPHPQKETDKGAVRSVRRFLQ
EAEIRP

Nucleotide

• Length: 261 bp

>T196623 NZ_CP095234:1075889-1076107 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12085.50 Da
• Isoelectric Point: 4.4537

>AT196623 WP_204347447.1 NZ_CP071626:c2420664-2420335 [Rhizobium leguminosarum]
MNVMHYKGYEAVIEFDEEAEIFHGEVVNMRDVITFQGTSAAELKQAFADSLDDYLAFCAERGEQPEKPFSGQFVVRADPT
LHKALSSAAQRDGVSLNKWVTSALERAVG

Nucleotide

• Length: 330 bp

>AT196623 NZ_CP095234:1075488-1075862 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A6N9ZKV3