Detailed information of TA system TA196620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 518952..519583
• Replicon: chromosome
• Accession: NZ_CP071626
• Organism: Rhizobium leguminosarum strain GLR17

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J3P71_RS02575
• Protein ID: WP_168283950.1
• Coordinates: 518952..519344 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4R0AC40
• Locus tag: J3P71_RS02580
• Protein ID: WP_020052605.1
• Coordinates: 519341..519583 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3P71_RS02550	514524..515288	-	765	WP_168304496.1	SDR family oxidoreductase	-
J3P71_RS02555	515415..516320	+	906	WP_168304495.1	LysR family transcriptional regulator	-
J3P71_RS02560	516322..517200	-	879	WP_168304494.1	LysR family transcriptional regulator	-
J3P71_RS02565	517291..518541	+	1251	WP_168304493.1	MFS transporter	-
J3P71_RS02570	518538..518924	+	387	WP_168283951.1	VOC family protein	-
J3P71_RS02575	518952..519344	-	393	WP_168283950.1	type II toxin-antitoxin system VapC family toxin	Toxin
J3P71_RS02580	519341..519583	-	243	WP_020052605.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J3P71_RS02585	519637..521298	-	1662	WP_168304492.1	urocanate hydratase	-
J3P71_RS02590	521316..521645	-	330	WP_168304491.1	TfoX/Sxy family protein	-
J3P71_RS02595	521649..522746	-	1098	WP_168304490.1	DUF917 domain-containing protein	-
J3P71_RS02600	522727..524007	-	1281	WP_168304489.1	Zn-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-121)

Antitoxin

• SpoVT-AbrB domain (7-43)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14557.58 Da
• Isoelectric Point: 5.7723

>T196620 WP_168283950.1 NZ_CP071626:c519344-518952 [Rhizobium leguminosarum]
MKYLLDANAVIALMKGNERFVAELRKHRPQDFGVPAIVAHELFYGAYKSLRTDENLARIDALQFNILEFDRDDARKAGEI
RAALQASGTPIGPYDVLIAGQAAARGLILITRNLREFERAADLQTENWEG

Nucleotide

• Length: 393 bp

>T196620 NZ_CP095186:3292406-3292660 [Pseudoxanthomonas mexicana]
GTGGACAACCGCGCGGCCGCCATCGAACGGGTCCGGCGCGTATTCGCGCATGTGGACCACCTGGCGGATCATCCCGACAG
CGGCCGCCGACCTCCTGAGCTGAAGCGCGGCCGCTATCGCCAGATCGTCGAACCGCCGTGCCGCGTGTTCCATCGATCAG
AGCCGGGGCGCGTGCTCATCCTGCATGTCATGCGCAGCGAGCGCCTGCTGCGGCCCGGCATGCTGCAAGCCCGCAAACCG
GGCAAGGCGCGCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9051.12 Da
• Isoelectric Point: 4.3322

>AT196620 WP_020052605.1 NZ_CP071626:c519583-519341 [Rhizobium leguminosarum]
METAKIFWSGRSQAVRLPKEFRFDGDSVAIRRQGRAIILEPISGDWEWLADVTGPVDPDFATAAEEQPAGQERPELDFFK

Nucleotide

• Length: 243 bp

>AT196620 NZ_CP095186:3292095-3292352 [Pseudoxanthomonas mexicana]
ATGCGCACCGAACTCGTCACCACGCTGAAGCGCCAGGCCACCGGGCTGCTGGCCGACGTCGAACGGGATCGCCAGCCGAT
CCTGATCACCCAGCACGGATTGCCCAGCGCGTATCGGGTCGACGTGGAAACCTGCGAAACGCTGCTGTCGCGCATGGCCG
TGCTGGAAGGCATCGCGCGCGGCGAGCAGGCCGTGGCCGAAGGTCGGGCGGTCGCCCATCCTCAAGCCAGGGACCGGCTT
GCCAGAGGGTTGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A4R0AC40