Detailed information of TA system TA196617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 333755..334410
• Replicon: plasmid p3
• Accession: NZ_CP071621
• Organism: Rhizobium bangladeshense strain PLR8-1a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J2J97_RS27345
• Protein ID: WP_207603870.1
• Coordinates: 333755..334174 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: J2J97_RS27350
• Protein ID: WP_127431704.1
• Coordinates: 334174..334410 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2J97_RS27320	329888..330130	+	243	WP_207603868.1	helix-turn-helix domain-containing protein	-
J2J97_RS27325	330504..331454	+	951	WP_207603869.1	pyridoxal-phosphate dependent enzyme	-
J2J97_RS27330	332045..332479	-	435	WP_207603986.1	ACT domain-containing protein	-
J2J97_RS27335	332782..333257	-	476	Protein_315	ABC transporter permease subunit	-
J2J97_RS27340	333251..333607	+	357	Protein_316	IS6 family transposase	-
J2J97_RS27345	333755..334174	-	420	WP_207603870.1	PIN domain-containing protein	Toxin
J2J97_RS27350	334174..334410	-	237	WP_127431704.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J2J97_RS27355	335516..335986	+	471	WP_207603871.1	redoxin domain-containing protein	-
J2J97_RS27360	336265..336960	+	696	WP_207603872.1	response regulator	-
J2J97_RS27365	337462..337596	-	135	Protein_321	transposase	-
J2J97_RS27370	337823..338002	-	180	Protein_322	IS3 family transposase	-
J2J97_RS27375	338345..338542	+	198	WP_041936667.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..382349	382349

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-117)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15503.75 Da
• Isoelectric Point: 5.1960

>T196617 WP_207603870.1 NZ_CP071621:c334174-333755 [Rhizobium bangladeshense]
MARYMLDTNMCIYLMKNQPEQVARRFASCYVGDVVMSAITFAELGYGVSASDDPARELDNLTALAELITVEPFGIAAARA
YGPVRMATRDRKIDHLDKLIASHAIALDTVLVTNNTRDFLAHPGLKLENWLDAERRETN

Nucleotide

• Length: 420 bp

>T196617 NZ_CP095181:76340-76822 [Enterobacter roggenkampii]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTGCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9065.18 Da
• Isoelectric Point: 5.6514

>AT196617 WP_127431704.1 NZ_CP071621:c334410-334174 [Rhizobium bangladeshense]
MHTTKAFKNGNSQAVRIPAELAFERTDVELEIERIGDELRIRPVHRSLAGVLDVFARFSPDFMRDGRESHEQEERDKI

Nucleotide

• Length: 237 bp

>AT196617 NZ_CP095181:76086-76352 [Enterobacter roggenkampii]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA