Detailed information of TA system TA196615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 516105..516640
• Replicon: plasmid p1
• Accession: NZ_CP071619
• Organism: Rhizobium bangladeshense strain PLR8-1a

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: J2J97_RS23155
• Protein ID: WP_207603528.1
• Coordinates: 516365..516640 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A5R9N071
• Locus tag: J2J97_RS23150
• Protein ID: WP_064709638.1
• Coordinates: 516105..516365 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2J97_RS23130	511281..511607	+	327	WP_064713213.1	DUF2934 domain-containing protein	-
J2J97_RS23135	511612..512031	+	420	WP_138396564.1	HD domain-containing protein	-
J2J97_RS23140	512207..514603	+	2397	WP_207608938.1	PAS-domain containing protein	-
J2J97_RS23145	514819..516015	-	1197	Protein_482	DUF2264 domain-containing protein	-
J2J97_RS23150	516105..516365	+	261	WP_064709638.1	ribbon-helix-helix domain-containing protein	Antitoxin
J2J97_RS23155	516365..516640	+	276	WP_207603528.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J2J97_RS23160	516647..517654	-	1008	WP_207603276.1	substrate-binding domain-containing protein	-
J2J97_RS23165	517863..519182	+	1320	WP_207608939.1	alpha-L-fucosidase	-
J2J97_RS23170	519231..520454	+	1224	WP_207603278.1	extracellular solute-binding protein	-
J2J97_RS23175	520523..521449	+	927	WP_207603279.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..622307	622307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10929.58 Da
• Isoelectric Point: 10.2902

>T196615 WP_207603528.1 NZ_CP071619:516365-516640 [Rhizobium bangladeshense]
MEVKWTRKAVSDIARLYDFLSPVDRRAAARTVQALTAAPARLMEQPRLGERLEEFDPREVRRILVGRYELRYEIQQSTIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T196615 NZ_CP095180:c3985731-3985321 [Enterobacter roggenkampii]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTTGCGGC
CATTGTGTTACTGATGCCGTGGCCGCTGAGCTATACGCCGCTGTGGCTGCTGTTACTGTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGTATTAATGCCCGTCAGGGAGAGATTAAATTACTGATGGATTCCCGCCTGCGCTGGCAGGGAAAA
GAGTGGGATATTCTCGGGATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGTTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTTTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGAGACCTGCGCCGGATGCTGTTGCAACAGACGA
CGCAGGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9729.02 Da
• Isoelectric Point: 4.6135

>AT196615 WP_064709638.1 NZ_CP071619:516105-516365 [Rhizobium bangladeshense]
METKVLTAHVPLPLAQKVDQLAMRLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDGGNIVDHQSVQAWADSLDSDE
PISLPR

Nucleotide

• Length: 261 bp

>AT196615 NZ_CP095180:c3985978-3985712 [Enterobacter roggenkampii]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACACCTTAAGCGACGACGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A5R9N071