Detailed information of TA system TA196614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 241567..242252
• Replicon: plasmid p4
• Accession: NZ_CP071616
• Organism: Rhizobium bangladeshense strain BLR175

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J2J98_RS28915
• Protein ID: WP_207604045.1
• Coordinates: 241830..242252 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1C3Y1G2
• Locus tag: J2J98_RS28910
• Protein ID: WP_064709306.1
• Coordinates: 241567..241833 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2J98_RS28880	236714..237178	-	465	WP_064709300.1	Lrp/AsnC family transcriptional regulator	-
J2J98_RS28885	237341..238120	+	780	WP_207604040.1	ATP-binding cassette domain-containing protein	-
J2J98_RS28890	238169..238942	+	774	WP_207604041.1	transporter substrate-binding domain-containing protein	-
J2J98_RS28895	238955..239680	+	726	WP_207604042.1	ABC transporter permease subunit	-
J2J98_RS28900	239694..240383	+	690	WP_207604043.1	ABC transporter permease subunit	-
J2J98_RS28905	240460..241368	+	909	WP_207604044.1	homocysteine S-methyltransferase family protein	-
J2J98_RS28910	241567..241833	+	267	WP_064709306.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J2J98_RS28915	241830..242252	+	423	WP_207604045.1	PIN domain-containing protein	Toxin
J2J98_RS28920	242329..243099	-	771	WP_138395988.1	ABC transporter ATP-binding protein	-
J2J98_RS28925	243109..244092	-	984	WP_064712939.1	ABC transporter substrate-binding protein	-
J2J98_RS28930	244131..244982	-	852	WP_207604046.1	ABC transporter permease subunit	-
J2J98_RS28935	244993..246345	-	1353	WP_207604047.1	NtaA/DmoA family FMN-dependent monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..285863	285863

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-60)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15388.58 Da
• Isoelectric Point: 4.9493

>T196614 WP_207604045.1 NZ_CP071616:241830-242252 [Rhizobium bangladeshense]
VRLLLDTNVLSEVTKPNPDVRVLGWLGGLDEDRSFISVVSVAEIRRGVTLMDNGRKRDVLADWLARDLPQRFERRIIPVD
EAVALAWGDLMGHAKRNGRGLSSMDGLIAATAIAHDLTLATRNTGDFEGFGIELVDPWVG

Nucleotide

• Length: 423 bp

>T196614 NZ_CP095180:c3095736-3095404 [Enterobacter roggenkampii]
ATGGACAGAGGGGAAATTTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTAGTGGTTCCCGTCACCAGCGGCGGTAACTTTGCTCGCA
CGGCAGGTTTCACCGTATCACTGGAAGGGGCGGGGACAAAAACGATGGGTGTTATTCGCTGCGACCAGCCCAGAACTATT
GATATGGCTGCCCGGAACGGCATGCGGCTGGAACGCATACCTGACGCCGTTGTAAATGAGGTGCTTGCCAGGCTGAATGC
GATTCTGAGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9781.96 Da
• Isoelectric Point: 6.3615

>AT196614 WP_064709306.1 NZ_CP071616:241567-241833 [Rhizobium bangladeshense]
MQSSRQGDESWTVASAKAKLSEVIERAQSAPQTITRNGKPSVVVVSAEEWQRKIARKGTLAEFLMESPLRGADLDLERQR
DEPRDSGL

Nucleotide

• Length: 267 bp

>AT196614 NZ_CP095180:c3095994-3095737 [Enterobacter roggenkampii]
ATGTATACAACTCGTCTTAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAACT
CTCGACGGACAGCGAAGTCGGCATGACCATCAATAATGGCTGCCTGATTATCGAACCCCAGAAACGGCCTCATTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCTGAAATGAGCGAAGAGGATCGGGAATGGATGGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1C3Y1G2