Detailed information of TA system TA196613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 317229..317884
• Replicon: plasmid p3
• Accession: NZ_CP071615
• Organism: Rhizobium bangladeshense strain BLR175

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J2J98_RS27435
• Protein ID: WP_207603870.1
• Coordinates: 317229..317648 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: J2J98_RS27440
• Protein ID: WP_127431704.1
• Coordinates: 317648..317884 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2J98_RS27410	313371..313613	+	243	WP_207603868.1	helix-turn-helix domain-containing protein	-
J2J98_RS27415	313986..314936	+	951	WP_207603869.1	pyridoxal-phosphate dependent enzyme	-
J2J98_RS27420	315527..315961	-	435	WP_207603986.1	ACT domain-containing protein	-
J2J98_RS27425	316264..316739	-	476	Protein_271	ABC transporter permease subunit	-
J2J98_RS27430	316733..317081	+	349	Protein_272	IS6 family transposase	-
J2J98_RS27435	317229..317648	-	420	WP_207603870.1	PIN domain-containing protein	Toxin
J2J98_RS27440	317648..317884	-	237	WP_127431704.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J2J98_RS27445	318990..319460	+	471	WP_207603871.1	redoxin domain-containing protein	-
J2J98_RS27450	319739..320434	+	696	WP_207603872.1	response regulator	-
J2J98_RS27455	320936..321070	-	135	Protein_277	transposase	-
J2J98_RS27460	321366..322391	+	1026	WP_207603971.1	IS110 family transposase	-
J2J98_RS27465	322655..322834	-	180	Protein_279	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..369739	369739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-117)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15503.75 Da
• Isoelectric Point: 5.1960

>T196613 WP_207603870.1 NZ_CP071615:c317648-317229 [Rhizobium bangladeshense]
MARYMLDTNMCIYLMKNQPEQVARRFASCYVGDVVMSAITFAELGYGVSASDDPARELDNLTALAELITVEPFGIAAARA
YGPVRMATRDRKIDHLDKLIASHAIALDTVLVTNNTRDFLAHPGLKLENWLDAERRETN

Nucleotide

• Length: 420 bp

>T196613 NZ_CP095180:c2929750-2929647 [Enterobacter roggenkampii]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9065.18 Da
• Isoelectric Point: 5.6514

>AT196613 WP_127431704.1 NZ_CP071615:c317884-317648 [Rhizobium bangladeshense]
MHTTKAFKNGNSQAVRIPAELAFERTDVELEIERIGDELRIRPVHRSLAGVLDVFARFSPDFMRDGRESHEQEERDKI

Nucleotide

• Length: 237 bp

>AT196613 NZ_CP095180:2929640-2929785 [Enterobacter roggenkampii]
AACAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT