Detailed information of TA system TA196610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 296777..298256
• Replicon: plasmid p4
• Accession: NZ_CP071608
• Organism: Rhizobium binae strain BLR195

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: J2J99_RS30555
• Protein ID: WP_127431472.1
• Coordinates: 296777..297646 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: J2J99_RS30560
• Protein ID: WP_127431473.1
• Coordinates: 297639..298256 (-)
• Length: 206 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2J99_RS30540	294207..294773	+	567	WP_168257339.1	conjugative transfer signal peptidase TraF	-
J2J99_RS30545	294763..295926	+	1164	WP_168301546.1	conjugal transfer protein TraB	-
J2J99_RS30550	295943..296550	+	608	Protein_265	conjugal transfer protein TraH	-
J2J99_RS30555	296777..297646	-	870	WP_127431472.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
J2J99_RS30560	297639..298256	-	618	WP_127431473.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
J2J99_RS30565	298603..298845	-	243	WP_127431474.1	helix-turn-helix domain-containing protein	-
J2J99_RS30570	299048..299392	+	345	WP_168259698.1	transcriptional repressor TraM	-
J2J99_RS30575	299396..300100	-	705	WP_127431476.1	autoinducer binding domain-containing protein	-
J2J99_RS30580	300413..301708	-	1296	WP_168301548.1	IncP-type conjugal transfer protein TrbI	-
J2J99_RS30585	301720..302160	-	441	WP_168301549.1	conjugal transfer protein TrbH	-
J2J99_RS30590	302164..302976	-	813	WP_168301550.1	P-type conjugative transfer protein TrbG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..312143	312143

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-263)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (15-56)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32569.35 Da
• Isoelectric Point: 5.0710

>T196610 WP_127431472.1 NZ_CP071608:c297646-296777 [Rhizobium binae]
MAKEIRNVGASVRARLLQLAKASGQSFDLVLTRFALERLLFRLSQSPHADRFVLKGAMLMMSWFDNPHRGTRDLDLLGFG
DPSPDPMLETFREILAQEADDGVTFDADTLHVDRIREALDYGGLRLRVITSISGARINLTIDIGFGDALEPGVEVLDYPS
MLDFPMPRLRAYARETVIAEKFQAMVMLGRVNSRMKDFYDIWILSRSFDFSDDRLARAIAATFDRRETPIPIDLPDALTD
AFAKDQQKQRQWRAFIEGVAHNPGDLIDVIAQIATFLMPHAVAAARLDE

Nucleotide

• Length: 870 bp

>T196610 NZ_CP095180:c314179-313877 [Enterobacter roggenkampii]
ATGAAGGAGATTGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAACTTAAAAGATCGACGAGCGAAGACCATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACGTTAAACCCGTGGGAGAAGGTATAAGTGAGCTAAGGATCC
ACTTTGGTCCGGGCTACAGGATCTATTTTAAAGACCAGGGCAATTACATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTGATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 206 a.a.
• Molecular weight: 22389.86 Da
• Isoelectric Point: 10.2141

>AT196610 WP_127431473.1 NZ_CP071608:c298256-297639 [Rhizobium binae]
MPSSVTQRQIARTVLTERGIARFVELRNAGVTAATMSRMERDGEVLRLARGLYQLPDAQLDASHSLAEVAKRAPKAVICL
VSALAFHSLTDQLPGQIWLAIGRKDWPPKLEAPAVRIVRFTDSLLNDSVETHIIEGVPVRVFGIAKTIADCFRYRNKIGL
SVAIEGLQEALRQRRATPGEIANQAERGGVGSVIRPYLEALTANG

Nucleotide

• Length: 618 bp

>AT196610 NZ_CP095180:c313870-313583 [Enterobacter roggenkampii]
ATGCATAAATTAACACCTTACGATCCCGCCAACGCGTTGGTGGATGACGAGGAAATCGCCGTGTTTATCGCTGATGCACT
AGAAACAGGCGACTCGACATATATCGCTAAAGCCCTTGGTGTTGTCGCGCGAGCCAAAGGCATGTCGAACATTTCACAGC
AAACCGGTTTATCACGTGAGCAGCTTTATCGGTCATTCAGTGATAAAGGAAACCCAACGCTCAAAACCACGCTCGCGGTC
ATGAAAGCATTGGGTTTTGGGCTAACACTCAAACACGCCGGGGATTAA