Detailed information of TA system TA196609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 83856..84511
• Replicon: plasmid p4
• Accession: NZ_CP071608
• Organism: Rhizobium binae strain BLR195

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A432N9U1
• Locus tag: J2J99_RS29590
• Protein ID: WP_018496488.1
• Coordinates: 83856..84275 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: J2J99_RS29595
• Protein ID: WP_168302099.1
• Coordinates: 84275..84511 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2J99_RS29570	79662..80532	+	871	Protein_69	sugar ABC transporter substrate-binding protein	-
J2J99_RS29575	81092..81325	+	234	WP_131702551.1	helix-turn-helix domain-containing protein	-
J2J99_RS29580	82148..82582	-	435	WP_131702666.1	ACT domain-containing protein	-
J2J99_RS29585	83383..83708	+	326	Protein_72	IS6 family transposase	-
J2J99_RS29590	83856..84275	-	420	WP_018496488.1	type II toxin-antitoxin system VapC family toxin	Toxin
J2J99_RS29595	84275..84511	-	237	WP_168302099.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J2J99_RS29600	84869..85165	+	297	WP_168302100.1	hypothetical protein	-
J2J99_RS29605	85615..87201	-	1587	WP_207600943.1	IS66 family transposase	-
J2J99_RS29610	87253..87606	-	354	WP_168302461.1	IS66 family insertion sequence element accessory protein TnpB	-
J2J99_RS29615	87587..88009	-	423	WP_168302462.1	transposase	-
J2J99_RS29620	88499..89232	+	734	Protein_79	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..312143	312143

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-117)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15616.86 Da
• Isoelectric Point: 4.9496

>T196609 WP_018496488.1 NZ_CP071608:c84275-83856 [Rhizobium binae]
MARYMLDTNMCIYLMKNQPEQVARRFASCYVGDVVMSAITFAELEYGVSASDDPARELDNLTALAELITVEPFGIAAARA
YGPVRMATRDRKKDHLDKLIASHAIALDTVLVTNNTRDFLAYPGLKLENWLDAERRETN

Nucleotide

• Length: 420 bp

>T196609 NZ_CP095180:c313870-313583 [Enterobacter roggenkampii]
ATGCATAAATTAACACCTTACGATCCCGCCAACGCGTTGGTGGATGACGAGGAAATCGCCGTGTTTATCGCTGATGCACT
AGAAACAGGCGACTCGACATATATCGCTAAAGCCCTTGGTGTTGTCGCGCGAGCCAAAGGCATGTCGAACATTTCACAGC
AAACCGGTTTATCACGTGAGCAGCTTTATCGGTCATTCAGTGATAAAGGAAACCCAACGCTCAAAACCACGCTCGCGGTC
ATGAAAGCATTGGGTTTTGGGCTAACACTCAAACACGCCGGGGATTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9141.27 Da
• Isoelectric Point: 5.6514

>AT196609 WP_168302099.1 NZ_CP071608:c84511-84275 [Rhizobium binae]
MHTTKAFKNGNSQAVRIPAELAFERTDVELEIERIGDELRIRPVHRSLAGVLDVFARFYPDFMRDGRESHEQEERDKI

Nucleotide

• Length: 237 bp

>AT196609 NZ_CP095180:c314179-313877 [Enterobacter roggenkampii]
ATGAAGGAGATTGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAACTTAAAAGATCGACGAGCGAAGACCATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACGTTAAACCCGTGGGAGAAGGTATAAGTGAGCTAAGGATCC
ACTTTGGTCCGGGCTACAGGATCTATTTTAAAGACCAGGGCAATTACATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTGATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A432N9U1