Detailed information of TA system TA196608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 431089..431855
• Replicon: plasmid p3
• Accession: NZ_CP071607
• Organism: Rhizobium binae strain BLR195

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: J2J99_RS28940
• Protein ID: WP_168300428.1
• Coordinates: 431089..431571 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: J2J99_RS28945
• Protein ID: WP_168300488.1
• Coordinates: 431571..431855 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2J99_RS28915	426659..427429	+	771	WP_168300490.1	glucose 1-dehydrogenase	-
J2J99_RS28920	427543..428442	-	900	WP_168300489.1	LysR family transcriptional regulator	-
J2J99_RS28925	428562..429425	+	864	WP_168300430.1	NmrA family NAD(P)-binding protein	-
J2J99_RS28930	429550..430409	-	860	Protein_401	NmrA family NAD(P)-binding protein	-
J2J99_RS28935	430579..431013	+	435	WP_168300429.1	hypothetical protein	-
J2J99_RS28940	431089..431571	-	483	WP_168300428.1	GNAT family N-acetyltransferase	Toxin
J2J99_RS28945	431571..431855	-	285	WP_168300488.1	DUF1778 domain-containing protein	Antitoxin
J2J99_RS28950	432279..432782	-	504	WP_168300427.1	SRPBCC family protein	-
J2J99_RS28955	432775..433122	-	348	WP_168300426.1	helix-turn-helix domain-containing protein	-
J2J99_RS28960	433290..433799	-	510	WP_168300425.1	nucleoside kinase	-
J2J99_RS28965	434050..434635	-	586	Protein_408	methyltransferase domain-containing protein	-
J2J99_RS28970	434671..435540	-	870	WP_168300424.1	hypothetical protein	-
J2J99_RS28975	435568..436107	-	540	WP_168300423.1	flavin reductase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..492894	492894

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17169.82 Da
• Isoelectric Point: 9.0487

>T196608 WP_168300428.1 NZ_CP071607:c431571-431089 [Rhizobium binae]
MLLPPALLGEAHDLQLFDSGHDSLDEWLRRRARANQVSGASRTYVVCEGKRVIGYYCLSSGALAVADAPGPVRRNMPDPV
PMAVLGRLAIDRSWQGKGIGAALLQDAVLRTGQAAHIMGIRGLLVHAISDEAKAFYEHYGFVASPANPVTLVLSLKARQA

Nucleotide

• Length: 483 bp

>T196608 NZ_CP095180:c78203-77826 [Enterobacter roggenkampii]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCACGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10620.95 Da
• Isoelectric Point: 8.6280

>AT196608 WP_168300488.1 NZ_CP071607:c431855-431571 [Rhizobium binae]
MASKNATDTHKHAVNLKVRDDIRGLIDRAAKAHGKTRSDFMIDAARRAAEDALLDQTLVQVDHDSYKHYLAVLDQPPSGE
GFERLMNAPRPWQR

Nucleotide

• Length: 285 bp

>AT196608 NZ_CP095180:c78619-78293 [Enterobacter roggenkampii]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCTTCGGCTCCTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAA
AAAGTAA