Detailed information of TA system TA196606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 297860..298467
• Replicon: plasmid p2
• Accession: NZ_CP071606
• Organism: Rhizobium binae strain BLR195

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: J0VFB1
• Locus tag: J2J99_RS25935
• Protein ID: WP_003572077.1
• Coordinates: 298213..298467 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J2J99_RS25930
• Protein ID: WP_054185827.1
• Coordinates: 297860..298216 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2J99_RS25910	292871..293185	+	315	WP_168302177.1	transposase	-
J2J99_RS25915	294161..295332	+	1172	Protein_300	IS701 family transposase	-
J2J99_RS25920	295482..296708	-	1227	Protein_301	IS3 family transposase	-
J2J99_RS25925	296764..297451	+	688	Protein_302	transposase	-
J2J99_RS25930	297860..298216	-	357	WP_054185827.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J2J99_RS25935	298213..298467	-	255	WP_003572077.1	type II toxin-antitoxin system HicA family toxin	Toxin
J2J99_RS25940	298774..299025	+	252	WP_168302176.1	antitoxin	-
J2J99_RS25945	299660..300886	+	1227	WP_207600983.1	DUF4143 domain-containing protein	-
J2J99_RS25950	301130..302509	-	1380	WP_168302174.1	flagellar biosynthesis protein FlgA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..498121	498121

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9336.70 Da
• Isoelectric Point: 9.8124

>T196606 WP_003572077.1 NZ_CP071606:c298467-298213 [Rhizobium binae]
MNSKQRKTLAAVFKDPVSGTIEWAAIENLLVAAGADLIEGNGSRVKFEKDGIIASFHRPHPDKEAKRYQVRDAREFLMQI
GVTP

Nucleotide

• Length: 255 bp

>T196606 NZ_CP095178:c57391-56975 [Enterobacter hormaechei]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13288.93 Da
• Isoelectric Point: 4.9838

>AT196606 WP_054185827.1 NZ_CP071606:c298216-297860 [Rhizobium binae]
MNIMTYNGYHARIEFDAEDDVFFGRIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAADHYAEARLPA

Nucleotide

• Length: 357 bp

>AT196606 NZ_CP095178:c57618-57388 [Enterobacter hormaechei]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTAG
ACAGGGCCGATCCGGACTTTATGGCGCAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Structures

Toxin

• AlphaFold DB: A0A246TAE7