Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 102225..102754 Replicon chromosome
Accession NZ_CP071594
Organism Staphylococcus aureus strain PNID0137

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag J3R87_RS00635 Protein ID WP_000621176.1
Coordinates 102225..102587 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag J3R87_RS00640 Protein ID WP_000948331.1
Coordinates 102584..102754 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3R87_RS00615 (99204) 99204..99974 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
J3R87_RS00620 (99949) 99949..100428 - 480 WP_001190825.1 anti-sigma B factor RsbW -
J3R87_RS00625 (100430) 100430..100756 - 327 WP_001052491.1 anti-sigma factor antagonist -
J3R87_RS00630 (100875) 100875..101876 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
J3R87_RS00635 (102225) 102225..102587 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J3R87_RS00640 (102584) 102584..102754 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
J3R87_RS00645 (102839) 102839..103987 - 1149 WP_001281140.1 alanine racemase -
J3R87_RS00650 (104053) 104053..104412 - 360 WP_000581197.1 holo-ACP synthase -
J3R87_RS00655 (104416) 104416..104907 - 492 WP_001286801.1 PH domain-containing protein -
J3R87_RS00660 (104900) 104900..106477 - 1578 WP_001294646.1 PH domain-containing protein -
J3R87_RS00665 (106470) 106470..106949 - 480 WP_001287082.1 hypothetical protein -
J3R87_RS00670 (107158) 107158..107718 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T196583 WP_000621176.1 NZ_CP071594:c102587-102225 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T196583 NZ_CP095165:c3861904-3861494 [Enterobacter roggenkampii]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTTGCGGC
CTTTGTTTTACTGATGCCGTGGCCGCTGAGCTACACGCCGCTGTGGCTGCTGCTACTTTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATCAAGTTACTGATGGATTCCCGCCTGCGCTGGCAGGGAAAA
GAGTGGGATATTCTCGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGCTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTTTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGAGACCTGCGCCGGATGCTATTGCAACAGACGA
CGCAGGGGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT196583 WP_000948331.1 NZ_CP071594:c102754-102584 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT196583 NZ_CP095165:c3862151-3861885 [Enterobacter roggenkampii]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACACCTTAAGCGACGACGATAAGCGTCTGTTTGTCCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References