Detailed information of TA system TA196582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/DinJ(antitoxin)
• Location: 2178367..2179000
• Replicon: chromosome
• Accession: NZ_CP071590
• Organism: Bifidobacterium longum subsp. longum strain KCTC 3421

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W6EYF0
• Locus tag: BLL421_RS09185
• Protein ID: WP_012577854.1
• Coordinates: 2178367..2178735 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BLL421_RS09190
• Protein ID: WP_207436617.1
• Coordinates: 2178716..2179000 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLL421_RS09145	2173504..2174304	+	801	WP_207436613.1	phage antirepressor KilAC domain-containing protein	-
BLL421_RS09150	2174853..2175368	+	516	WP_207436614.1	hypothetical protein	-
BLL421_RS09155	2175365..2175631	+	267	WP_041473844.1	hypothetical protein	-
BLL421_RS09160	2175780..2176997	+	1218	WP_207436615.1	transposase	-
BLL421_RS09165	2177193..2177441	-	249	WP_015713507.1	hypothetical protein	-
BLL421_RS09170	2177550..2177696	-	147	WP_207436799.1	hypothetical protein	-
BLL421_RS09175	2177693..2177920	-	228	WP_207436616.1	hypothetical protein	-
BLL421_RS09180	2177917..2178180	-	264	WP_015713504.1	hypothetical protein	-
BLL421_RS09185	2178367..2178735	-	369	WP_012577854.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BLL421_RS09190	2178716..2179000	-	285	WP_207436617.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BLL421_RS09195	2179072..2179374	-	303	WP_206822301.1	hypothetical protein	-
BLL421_RS09200	2179460..2179642	-	183	WP_052789116.1	hypothetical protein	-
BLL421_RS09205	2179642..2179983	-	342	WP_200407903.1	hypothetical protein	-
BLL421_RS09210	2179986..2180732	-	747	WP_207436618.1	hypothetical protein	-
BLL421_RS09215	2180930..2181271	-	342	WP_207436619.1	hypothetical protein	-
BLL421_RS09220	2181387..2181731	-	345	WP_207436620.1	hypothetical protein	-
BLL421_RS09225	2181728..2181889	-	162	WP_155245937.1	hypothetical protein	-
BLL421_RS09230	2181959..2182171	-	213	WP_069483789.1	hypothetical protein	-
BLL421_RS09235	2182292..2182480	-	189	WP_032742291.1	helix-turn-helix domain-containing protein	-
BLL421_RS09240	2182673..2182942	-	270	WP_015713494.1	hypothetical protein	-
BLL421_RS09245	2182956..2183222	-	267	WP_015713493.1	hypothetical protein	-
BLL421_RS09250	2183373..2183600	-	228	WP_041473841.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2152276..2214677	62401

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (16-111)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-53)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13866.75 Da
• Isoelectric Point: 4.5746

>T196582 WP_012577854.1 NZ_CP071590:c2178735-2178367 [Bifidobacterium longum subsp. longum]
MTSTPSEPRLYDVWLMWVEFPDHPGIGKPRPVVITEVDGDLVSGIVAKITGNTDWDEAGDVPLLDWKAEGLLKPSLVRCS
QRFYFNRSELLQWFGRLSLRDAEHVNDGLEATLDIPPYRRSV

Nucleotide

• Length: 369 bp

>T196582 NZ_CP095165:c2889367-2889264 [Enterobacter roggenkampii]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10531.82 Da
• Isoelectric Point: 4.5582

>AT196582 WP_207436617.1 NZ_CP071590:c2179000-2178716 [Bifidobacterium longum subsp. longum]
MGKLVANIDDDIKARAAALYDSMGMSLSTAVNMFLRQSLVDNGLPFKPTRHTPDGYPVPPVHNAYMFERSEKGHVILPAD
WDDSEDDVYDQYAK

Nucleotide

• Length: 285 bp

>AT196582 NZ_CP095165:2889259-2889402 [Enterobacter roggenkampii]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Structures

Toxin

• AlphaFold DB: W6EYF0