Detailed information of TA system TA196581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/PIN-RelB
• Location: 1869711..1870564
• Replicon: chromosome
• Accession: NZ_CP071590
• Organism: Bifidobacterium longum subsp. longum strain KCTC 3421

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V6XWY1
• Locus tag: BLL421_RS07640
• Protein ID: WP_007054468.1
• Coordinates: 1870073..1870564 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V6XX41
• Locus tag: BLL421_RS07635
• Protein ID: WP_008783109.1
• Coordinates: 1869711..1870073 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLL421_RS07630	1866866..1869565	+	2700	WP_007054466.1	aconitate hydratase AcnA	-
BLL421_RS07635	1869711..1870073	+	363	WP_008783109.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BLL421_RS07640	1870073..1870564	+	492	WP_007054468.1	PIN domain-containing protein	Toxin
BLL421_RS07645	1870624..1872729	-	2106	WP_032747025.1	DUF262 domain-containing protein	-
BLL421_RS07650	1872819..1873460	-	642	WP_007052627.1	GNAT family N-acetyltransferase	-
BLL421_RS07655	1873500..1874225	-	726	WP_007056206.1	response regulator transcription factor	-
BLL421_RS07660	1874260..1875492	-	1233	WP_207436794.1	sensor histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-141)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (6-53)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 18328.91 Da
• Isoelectric Point: 4.5590

>T196581 WP_007054468.1 NZ_CP071590:1870073-1870564 [Bifidobacterium longum subsp. longum]
MTDVSMQRILLDTNVLLDYLLHRDDHAKMAEAVMELGAKNNITLLCASLSLKDIAYLSSSAIRREFKPNESEVENFTRGF
LSSRVPWRCIEQVKEMCDIVAVDESTCDKAFSLQKRHRDFEDDLIIVAAQQSGANCVVTSDAELISHFPEYCKTPAEIVA
ALE

Nucleotide

• Length: 492 bp

>T196581 NZ_CP095165:1714436-1714957 [Enterobacter roggenkampii]
GTGGACAATCTCACGATCGAAATGTTTTCAGAGGGAAAAGACTATGATTTCAGGGACTTCGATTGTGGTGAACCCTCGCT
AAATGTCTTCCTCACAGAACATCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGGCTATTTGCTTAAAGATCGCGATG
GTCCGCGGGTCCTGGGCTATTACACGTTGTCTGGTAGTTGCTTTGAAAAAGCGATGCTGCCTTCTAAGACTCAGCAGCGT
CGGATCCCTTATAGCAACGTACCGAGCGTAACGCTGGGACGTCTGGCGATACATAAAGACTTACAGGGGCTGGAGTGGGG
AACGACGCTCGTCACACATGCCATGCGGGTTGTCTATCTGGCATCGCAGGCGGTGGGTGTACACGGAATATTTGTTGATG
CACTCAACGACCAGGCAAAACAATTTTATCTGAAGCAGGGCTTTATTCCATTGACGGATGAAAACAGCCATTCTCTTTTC
TTTCCTACAAAATCTATCGAACGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13974.03 Da
• Isoelectric Point: 5.0733

>AT196581 WP_008783109.1 NZ_CP071590:1869711-1870073 [Bifidobacterium longum subsp. longum]
MTMVQMNVRIDAKLKNEVEEVLKGKGVSVSDVIRSLWLYIADRKEVPALETTAEEQAREEEKQRKLKLIREGAGYVHKEL
VKAGLISEDTDLMEGLTYKQFRDKMYDEQLDAYYNMKRAD

Nucleotide

• Length: 363 bp

>AT196581 NZ_CP095165:1714160-1714429 [Enterobacter roggenkampii]
ATGCCAGCACTGAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACGAATCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGGGCAGCGGAAGTCATCGAGCAGCACCGCCGTC
TCGTATTAAACGAAGCATCCTGGAATGCGGTGATGGATGCCATCGACAATCCACCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Structures

Toxin

• AlphaFold DB: V6XWY1

Antitoxin

• AlphaFold DB: V6XX41