Detailed information of TA system TA196576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 1992061..1992574
• Replicon: chromosome
• Accession: NZ_CP071589
• Organism: Staphylococcus simiae strain 17941E

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: J3R86_RS09160
• Protein ID: WP_207517036.1
• Coordinates: 1992061..1992270 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: G5JKX9
• Locus tag: J3R86_RS09165
• Protein ID: WP_002464830.1
• Coordinates: 1992281..1992574 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3R86_RS09140	1987577..1988935	-	1359	WP_207517033.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
J3R86_RS09145	1988950..1990020	-	1071	WP_207517034.1	D-alanine--D-alanine ligase	-
J3R86_RS09150	1990337..1991536	+	1200	WP_207517035.1	FtsW/RodA/SpoVE family cell cycle protein	-
J3R86_RS09155	1991801..1991938	-	138	WP_000828354.1	hypothetical protein	-
J3R86_RS09160	1992061..1992270	-	210	WP_207517036.1	cation transporter	Toxin
J3R86_RS09165	1992281..1992574	-	294	WP_002464830.1	copper-sensing transcriptional repressor CsoR	Antitoxin
J3R86_RS09170	1992745..1994232	+	1488	WP_207517037.1	cardiolipin synthase	-
J3R86_RS09175	1994257..1994907	+	651	WP_207517038.1	HD domain-containing protein	-
J3R86_RS09180	1995235..1996953	+	1719	Protein_1760	IS1182 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (18-67)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7612.51 Da
• Isoelectric Point: 4.0852

>T196576 WP_207517036.1 NZ_CP071589:c1992270-1992061 [Staphylococcus simiae]
MLNNSVIHTAGIETEEQAQHVSAILKEMIGVKSANVNIIDGSVEVEYETPSNLDSIEKEIYDAGYKVIY

Nucleotide

• Length: 210 bp

>T196576 NZ_CP095165:170361-170546 [Enterobacter roggenkampii]
GTGAAAAGTGCGGATGTCATTACTGTTCTGATCAGCCATGGCTGGAAATGTGTCAGAACGAAAGGAAGCCATCATCAGTT
TCGCCATCCCGAACAGAAAGGGCTGGTTACTGTCCCACATCCCAAGAAGGATATTAAACCGGGCACTCTCGCCCAGATCT
GGCGACAGGCGGGTATTAAACACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11160.73 Da
• Isoelectric Point: 5.6326

>AT196576 WP_002464830.1 NZ_CP071589:c1992574-1992281 [Staphylococcus simiae]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEDDVYCDDVLTQIRATRSALNSVATVLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLMKD

Nucleotide

• Length: 294 bp

>AT196576 NZ_CP095165:170561-170968 [Enterobacter roggenkampii]
ATGTTTTATCCGGCTTACATTCATTCTGACTCCGACGGTAGCGCCAGCGGCTTTTTCCCTGACGTTCCAGGCTGTTTTTT
TGCAGGTGATTCCCTGGACGACGCTTTTCAGGATGCGCGTGATGCGCTCACAGCACACTTCGAAGCGCTATTTGAAATGG
ATAATGCACTGCCTCTTCCGGGTAATGTGGAAGCGCATCTGGAAAGTCGTCCTGAGGATTTCACTGGCGGGCAATGGCTG
TTGGTAGATATTAATATGAAACAGTTCGACGGTAGGGCCGAGCGTATCAACATCACGATGCCTCGACGTTTGCTGGTAAA
AATTGATTCCTTTGTCAGCGAGCATCCTCAGTTCGGTAATCGAAGCGCATTTCTGGCTGAGGCAGCACGTCGCGTGCTGC
CTGGGTAA

Structures

Antitoxin

• AlphaFold DB: G5JKX9