Detailed information of TA system TA196575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1980015..1980547
• Replicon: chromosome
• Accession: NZ_CP071589
• Organism: Staphylococcus simiae strain 17941E

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G5JLV8
• Locus tag: J3R86_RS09095
• Protein ID: WP_002465156.1
• Coordinates: 1980015..1980380 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G5JLV7
• Locus tag: J3R86_RS09100
• Protein ID: WP_002465155.1
• Coordinates: 1980377..1980547 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3R86_RS09075	1976743..1977513	-	771	WP_207517023.1	RNA polymerase sigma factor SigB	-
J3R86_RS09080	1977488..1977967	-	480	WP_207517024.1	anti-sigma B factor RsbW	-
J3R86_RS09085	1977969..1978295	-	327	WP_207517025.1	anti-sigma factor antagonist	-
J3R86_RS09090	1978413..1979414	-	1002	WP_207517026.1	SpoIIE family protein phosphatase	-
J3R86_RS09095	1980015..1980380	-	366	WP_002465156.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J3R86_RS09100	1980377..1980547	-	171	WP_002465155.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
J3R86_RS09105	1980632..1981780	-	1149	WP_207517027.1	alanine racemase	-
J3R86_RS09110	1981844..1982200	-	357	WP_207517028.1	holo-ACP synthase	-
J3R86_RS09115	1982242..1982721	-	480	WP_207517029.1	PH domain-containing protein	-
J3R86_RS09120	1982708..1984264	-	1557	WP_207517030.1	PH domain-containing protein	-
J3R86_RS09125	1984257..1984736	-	480	WP_207517031.1	PH domain-containing protein	-
J3R86_RS09130	1985053..1985364	+	312	Protein_1750	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1985176..1985364	188

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13514.85 Da
• Isoelectric Point: 10.2631

>T196575 WP_002465156.1 NZ_CP071589:c1980380-1980015 [Staphylococcus simiae]
MIKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSESKMKEVDNALIISLGLTTLSQHKLS

Nucleotide

• Length: 366 bp

>T196575 NZ_CP095165:48530-48928 [Enterobacter roggenkampii]
ATGAGACATATGCTGGATACGAATATTGTGAGTCACTTGTTTAAACGACACCCGGAGGTTGTAAGCAGAATGACATGTCT
GGCGCCGGGTGACGTTTGTATCTCAAGCATTACCGAAGCGGAATTGCTGTATGGTGCTGACAAGAAGAAAAGCAGTAGAT
TGAAAGAAACAATCCGGGAGTTTCTCAATACCATCACGATTTGCGACTGGGACAGCGACGCCGCAGCAACGTACGGTGAA
CTCCGTGCAACCCTGGAAAAGAAAGGGAAAGTGATGGGGGATCTCGATCAACTGATCGCTGCACATGCTATCAGTCGGGG
AACAACGATCGTTACTAACGATCGTGCATTCAGAATGGTGCAGGAGCTTGCCGTCGAGGACTGGACGACAACGTTGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6223.72 Da
• Isoelectric Point: 3.8305

>AT196575 WP_002465155.1 NZ_CP071589:c1980547-1980377 [Staphylococcus simiae]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNESYLSSNSSNE

Nucleotide

• Length: 171 bp

>AT196575 NZ_CP095165:48267-48533 [Enterobacter roggenkampii]
ATGGAACGCACAGCAAAACTATTCAAAAAGGGTCAGAATCAGGCCGTTATGCTGCCTGCTGAATTCGCGTTTGATACGGA
GAGCGTCTACATCCGACGGGATGAGGAGGGGAATGTCGTTTTGGTGGCAAAACCTGTTCTAATAAAGAGCTGGGATAATT
TTCTCCGCATGCTGGCAGAAACGGACGTACCAGATACGTTTTTGAGCAAGGAGGAACGCAATCAGAGTGTTACGACAAGA
GACCCTCTGGATGGAGCATGCTCATGA

Structures

Toxin

• AlphaFold DB: G5JLV8

Antitoxin

• AlphaFold DB: G5JLV7