Detailed information of TA system TA196567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2090267..2090883
• Replicon: chromosome
• Accession: NZ_CP071586
• Organism: Pseudomonas germanica strain FIT28

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: J0G10_RS09260
• Protein ID: WP_095050205.1
• Coordinates: 2090267..2090479 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7Z2KRV3
• Locus tag: J0G10_RS09265
• Protein ID: WP_064390887.1
• Coordinates: 2090479..2090883 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G10_RS09235 (J0G10_09245)	2085442..2087430	+	1989	WP_220558332.1	heme lyase CcmF/NrfE family subunit	-
J0G10_RS09240 (J0G10_09250)	2087427..2087963	+	537	WP_093439831.1	DsbE family thiol:disulfide interchange protein	-
J0G10_RS09245 (J0G10_09255)	2087960..2088430	+	471	WP_220558333.1	cytochrome c-type biogenesis protein CcmH	-
J0G10_RS09250 (J0G10_09260)	2088427..2089629	+	1203	WP_220558334.1	c-type cytochrome biogenesis protein CcmI	-
J0G10_RS09255 (J0G10_09265)	2089655..2090059	+	405	WP_016987493.1	hypothetical protein	-
J0G10_RS09260 (J0G10_09270)	2090267..2090479	+	213	WP_095050205.1	type II toxin-antitoxin system HicA family toxin	Toxin
J0G10_RS09265 (J0G10_09275)	2090479..2090883	+	405	WP_064390887.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J0G10_RS09270 (J0G10_09280)	2090953..2092140	-	1188	WP_095050206.1	MFS transporter	-
J0G10_RS09275 (J0G10_09285)	2092382..2093002	+	621	WP_220558335.1	DUF5666 domain-containing protein	-
J0G10_RS09280 (J0G10_09290)	2093241..2094239	-	999	WP_007950301.1	sulfate ABC transporter substrate-binding protein	-
J0G10_RS09285 (J0G10_09295)	2094441..2095265	-	825	WP_007919942.1	ion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8044.22 Da
• Isoelectric Point: 10.3456

>T196567 WP_095050205.1 NZ_CP071586:2090267-2090479 [Pseudomonas germanica]
VQSRLLIKELEEAGWTLDRITGSHHIFRHRYNPYTIPVPHPKKDLPLGTVKSIRRRAGLYNPPASFEGDP

Nucleotide

• Length: 213 bp

>T196567 NZ_CP095162:c1441160-1440891 [Streptococcus suis]
ATGGCTTATAAGCTTGTTCTCAGCGATGATGCTTTGAAGCAGTTGAAAAAAATGGACAGGCATGTGGGGATGATGCTTGC
TAAGGATTTGAAAAAACGTTTGGACGGTTTGGAGAATCCAAGACAATTTGGGAAGGCCTTGGTAGGAGACTATAAGGGGC
TGTGGCGGTATAGAGTGGGGAATTACCGTGTAATTTGTGATATTATTGATAACAAAATGGTCATCCTGGCCCTTGAAATC
GGGCACCGGAAAGAAATCTATAAAAAATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14789.75 Da
• Isoelectric Point: 4.3849

>AT196567 WP_064390887.1 NZ_CP071586:2090479-2090883 [Pseudomonas germanica]
MQYPICIEWGDENTAIGIQIPDIPGAVTAGDSFEDAYKAAVEVAHIMLQEIAADGESIPMPTSASAHRSNPDFADMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRY

Nucleotide

• Length: 405 bp

>AT196567 NZ_CP095162:c1441377-1441150 [Streptococcus suis]
ATGGGAATTGTATCCTTGCGATTAAACGATGCAGAAGAAGAAATTTTCAGAAGCTATGCTATTCATACAGGAAAAAGCCT
GTCTGAGCTATTCAAAACTGCACTTGCTGAACAGATAGAAGACCAGTTGGACTATGAAATTGGTATCAAGGCTTTGAAGA
AATTCCAAGAAAATCCTGTTACACACTCGATTGATGATGTTCTTGCGGAGTTAGAAGATGGCTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7Z2KRV3