Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 25009..25534 Replicon plasmid p1_UPEC129
Accession NZ_CP071523
Organism Escherichia coli strain UPEC129

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag J3P56_RS24860 Protein ID WP_001159871.1
Coordinates 25229..25534 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag J3P56_RS24855 Protein ID WP_000813634.1
Coordinates 25009..25227 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3P56_RS24850 22046..22687 - 642 WP_000990667.1 hypothetical protein -
J3P56_RS24855 25009..25227 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
J3P56_RS24860 25229..25534 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
J3P56_RS24865 25535..26344 + 810 WP_000016979.1 site-specific integrase -
J3P56_RS24870 26482..26757 - 276 WP_000239529.1 hypothetical protein -
J3P56_RS24875 26751..27395 - 645 WP_000633911.1 AAA family ATPase -
J3P56_RS24880 27624..28595 + 972 WP_001103694.1 hypothetical protein -
J3P56_RS24885 28600..28992 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
J3P56_RS24890 28997..30267 - 1271 Protein_32 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..49605 49605


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T196562 WP_001159871.1 NZ_CP071523:25229-25534 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T196562 NZ_CP095162:765731-767008 [Streptococcus suis]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAGCTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAT
CTAAAGGTTATGAAACAAAAATATACGTCATGGCAGTACCGAAAATTGAATCATACTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AGCTAATAAAGACCGCCCTCAGTTACAAGCTATGCTTGCTTATATACGTGAAGGTGATATAGTGGTTGTGACAGAATTAG
AACGCCTGGGTAGGAATAACAAGGAGCTAACTGAGGTCATGAATGATATTCAAGCCAAGGGGGCAACCTTGGAAGTCTTA
AATCTTCCCACTCTTCGAGGGATTGAGGATGACAACCTGAGACGGCTCTTGAACAATCTTATCCTGGAACTCTACAAGTA
CCAGGCACAAGCTGAAAGGGAACGCATAAAGGAACGGCAACGCCAAGGGATAGCTATTGCCAAGACTCAAGGAAAGTACA
AAGGCAGAAAAGCCATTTTTTCAGAAGATGACAGCAGGCTTCTTCATGCCTTTGACCTTTACCTTGAAGGTCTATCAGAT
AAAGACGTTGCCAAGCTAACAGGTATCAACGAACGTACTTTTAGGCGGTATCGTGAAAAGCATAAGATAAAAAGATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT196562 WP_000813634.1 NZ_CP071523:25009-25227 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT196562 NZ_CP095162:765457-765729 [Streptococcus suis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References