Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4557581..4558078 Replicon chromosome
Accession NZ_CP071522
Organism Escherichia coli strain UPEC129

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag J3P56_RS22340 Protein ID WP_001173343.1
Coordinates 4557581..4557754 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag J3P56_RS22345 Protein ID WP_001312331.1
Coordinates 4557782..4558078 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3P56_RS22330 4554251..4555708 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
J3P56_RS22335 4555945..4557462 + 1518 WP_000003806.1 lysine--tRNA ligase -
J3P56_RS22340 4557581..4557754 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
J3P56_RS22345 4557782..4558078 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
J3P56_RS22350 4558306..4558578 - 273 WP_000405647.1 N-acetyltransferase -
J3P56_RS22355 4558590..4558820 - 231 WP_000373293.1 (4Fe-4S)-binding protein -
J3P56_RS22360 4559001..4560632 + 1632 WP_001216482.1 sensor histidine kinase -
J3P56_RS22365 4560629..4561348 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T196560 WP_001173343.1 NZ_CP071522:c4557754-4557581 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T196560 NZ_CP095161:1229786-1229968 [Avibacterium paragallinarum]
GTGAACAGTGCAAATTTGATAAAACAGGTTGAAAAGGATGGTTGGGTGCTTGTATCAGTGAAAGGAAGCCATCACCAATT
TAAGCACCCCACGAAGAAAGGACGCGTTACCATCCCACACCCGAAAAAAGATTTACACATAAAAACAGTAAATTCAATCT
TAAAACAAGCGGGGCTAAAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT196560 WP_001312331.1 NZ_CP071522:c4558078-4557782 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT196560 NZ_CP095161:1230005-1230412 [Avibacterium paragallinarum]
ATGCTATATCCAATCGGAATTGAAATGGGCGATGAAAAACACGCTTACGGCGTTGTTGTGCCTGATGTACCAGGTTGCTT
CTCTGCGGGTGATACGTTAGAAGAAGCCTTTACTAACGCAAAAGAAGCCATTGCATTTCATATTGAGGGAATGCTTGAAG
ATGGCGAAGAAATTCCTTTGCCAACTGATTTACAAAGACATATGCAAAATAAAGAGTATGAGGGATTTACCTTTACTGTG
GTTGATGTTGATCTTACCCACTTGATGGGCAAAGCTGAAAAAATTAATGTTACTCTACCAAAAATTTTAATCAACAAGAT
TGATAACTACGTTTCGACTCATCCACAATACAAAAGCCGTAGCGGTTTCCTCGCTCAAATCGCCACAGATAAATTATTAT
CCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References