Detailed information of TA system TA196559

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4545994..4546828
• Replicon: chromosome
• Accession: NZ_CP071522
• Organism: Escherichia coli strain UPEC129

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: J3P56_RS22295
• Protein ID: WP_000854820.1
• Coordinates: 4546451..4546828 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: J3P56_RS22290
• Protein ID: WP_001285610.1
• Coordinates: 4545994..4546362 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3P56_RS22255	4541715..4542395	+	681	WP_001278649.1	WYL domain-containing protein	-
J3P56_RS22260	4542546..4543223	+	678	WP_001097565.1	hypothetical protein	-
J3P56_RS22265	4543229..4543462	+	234	WP_000883181.1	DUF905 family protein	-
J3P56_RS22270	4543552..4544370	+	819	WP_001175155.1	DUF945 domain-containing protein	-
J3P56_RS22275	4544636..4545115	+	480	WP_000706978.1	antirestriction protein	-
J3P56_RS22280	4545130..4545606	+	477	WP_001186193.1	RadC family protein	-
J3P56_RS22285	4545693..4545914	+	222	WP_000692347.1	DUF987 domain-containing protein	-
J3P56_RS22290	4545994..4546362	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
J3P56_RS22295	4546451..4546828	+	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
J3P56_RS22300	4546825..4547022	+	198	Protein_4363	hypothetical protein	-
J3P56_RS22305	4547050..4547247	+	198	WP_095591310.1	DUF957 domain-containing protein	-
J3P56_RS22310	4547332..4547892	+	561	Protein_4365	DUF4942 domain-containing protein	-
J3P56_RS22315	4548727..4550265	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T196559 WP_000854820.1 NZ_CP071522:4546451-4546828 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T196559 NZ_CP095161:866146-866424 [Avibacterium paragallinarum]
ATGATTACTCACTTTACTTGTAAAGATACCCAAGCCTTTTTTGAAGGGCAACGCATTCGCCGCTTTATTCCATTTGAAAA
AGTCGCAATGCGAAAATTACAGCAACTTAATGCCGCAACAGAACTCGATTTTTTAAGGATTCCACCAGGGAATCATTTAG
AAATGCTGACTGGTGATAGAAAAGGACAATACAGTATTAGAATTAACGATCAATGGCGAATCTGCTTTACTTGGTTAAAC
GGACACGCTGCCGATGTAGCAATTGTGGATTACCACTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT196559 WP_001285610.1 NZ_CP071522:4545994-4546362 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT196559 NZ_CP095161:866434-866730 [Avibacterium paragallinarum]
ATGACAAGACAAATTCCCCTTTCTCACCCTGGCGAAATTTTGCTAGAAGAATGGCTAAAACCCTTAGGTATCAGCCAATA
TGCGTTAGCAAAAGCAATTTCTGTACCACCACGCCGAATCAATGAAATTGTCAAAGGAAAACGTGCGATCACACCAGACA
CGGCTTTACGCTTAGCCACCTATTTTGGGACAGATCCTCAAAGTTGGCTTAATCTCCAATCTCATTATGATACCGAGCAA
ACTCGTACCGCCATAAATGAAGATTTAACCCATATTTCTCCTTTTCAATTTGCCTAA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6