Detailed information of TA system TA196558

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4289923..4290757
• Replicon: chromosome
• Accession: NZ_CP071522
• Organism: Escherichia coli strain UPEC129

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: J3P56_RS21015
• Protein ID: WP_000854770.1
• Coordinates: 4289923..4290300 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: J3P56_RS21020
• Protein ID: WP_001332042.1
• Coordinates: 4290389..4290757 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3P56_RS20995	4288447..4288623	-	177	Protein_4107	helix-turn-helix domain-containing protein	-
J3P56_RS21000	4288987..4289145	-	159	WP_001467148.1	hypothetical protein	-
J3P56_RS21005	4289245..4289421	-	177	WP_000839286.1	DUF957 domain-containing protein	-
J3P56_RS21010	4289438..4289926	-	489	WP_000761690.1	hypothetical protein	-
J3P56_RS21015	4289923..4290300	-	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
J3P56_RS21020	4290389..4290757	-	369	WP_001332042.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
J3P56_RS21025	4290920..4291141	-	222	WP_000692323.1	DUF987 domain-containing protein	-
J3P56_RS21030	4291204..4291680	-	477	WP_001557203.1	RadC family protein	-
J3P56_RS21035	4291696..4292160	-	465	WP_000855061.1	antirestriction protein	-
J3P56_RS21040	4292502..4293320	-	819	WP_137571651.1	DUF945 domain-containing protein	-
J3P56_RS21045	4293475..4293633	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4278001..4314533	36532

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T196558 WP_000854770.1 NZ_CP071522:c4290300-4289923 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T196558 NZ_CP095161:563059-563253 [Avibacterium paragallinarum]
ATGGCGAAAGAATTTAAGCGCGATTTGGTACAACTAGCCAAAAAAGCACCGCACTTATTTGTTCATCCCCTTAAAAACAA
TTTGAAAGGTTATATGGATTGCCACATTCTCAATGATCTCGTGCTAATTTATAAAATTGAACAGCAATGCCTAAAATTAA
TTCGGCTAAATACACATTCAGAAGTTTTTGCATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13677.38 Da
• Isoelectric Point: 6.5931

>AT196558 WP_001332042.1 NZ_CP071522:c4290757-4290389 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT196558 NZ_CP095161:562735-563025 [Avibacterium paragallinarum]
ATGACGAACAATAATGCCGTGGTGAATTTTCGTTTACCACAACATTTAAAAACAGAAGCATTTGAAGTGATTGCCCAATA
TGGTCTTACCCCATCACAAGTATTTAATATGTTTTTAACTGAAATTGCTGCCACCAAAGCGATTCCTTTGAGTTTGAATT
ATTTACAACCTAATGCAAAAACTTTAGCGGCAATGAATGAAATTGAAAGTGGAACGGCTGAACGTCTTTCTCTTGATGAC
AAAACTGATCTTGCCACCTTATTGCAACAAATAGCAGAAGAGAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9