Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4219973..4220549 Replicon chromosome
Accession NZ_CP071522
Organism Escherichia coli strain UPEC129

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag J3P56_RS20670 Protein ID WP_001297643.1
Coordinates 4220262..4220549 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag J3P56_RS20665 Protein ID WP_000063156.1
Coordinates 4219973..4220275 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3P56_RS20650 4216569..4218719 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
J3P56_RS20655 4218769..4218972 + 204 WP_000467859.1 YbdD/YjiX family protein -
J3P56_RS20660 4218983..4219939 + 957 WP_001297640.1 GTPase -
J3P56_RS20665 4219973..4220275 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
J3P56_RS20670 4220262..4220549 - 288 WP_001297643.1 BrnT family toxin Toxin
J3P56_RS20675 4220748..4221445 - 698 Protein_4043 IS1 family transposase -
J3P56_RS20680 4221502..4221597 + 96 Protein_4044 dienelactone hydrolase family protein -
J3P56_RS20685 4221743..4222975 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
J3P56_RS20690 4223016..4224296 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4220748..4220957 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T196556 WP_001297643.1 NZ_CP071522:c4220549-4220262 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T196556 NZ_CP095161:c416419-416243 [Avibacterium paragallinarum]
GTGAACAGCAAACAAATGATAAAATTGCTCAAACAAGATGGTTGGTATCTTGACAGCATAAATGGAAGCCACCACCATTT
TGAGCATCATCAAAAAAAGGGAAAAGTTACCGTTCCCCACCCAAGAGCCGAATTAGGACATCTTGAAAAATTAATCAGAA
AACAAGCGGGGCTATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT196556 WP_000063156.1 NZ_CP071522:c4220275-4219973 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT196556 NZ_CP095161:c416221-415817 [Avibacterium paragallinarum]
ATGCTATATCCAATCGCACTTGAAAAAGTTACTGATGGTTATGTTGTCTCCGTGCCTGATATTCCTGGCTGTTTTTCAGC
TGGTGATACATTGGAAGAGGCATATAACAATGTAAAACAAGCTATCACATCACATCTGGAGTTAGTTGTCCGAGACAATG
AAGAAGTTCCTCTACCAACCCCGCTAGAAGAACATAAAGCCAATCCAGATTATCAAAGCGTTGATATTTTCTTTGGCGTC
ATTGATGTGGACATCAGCCACCTACTTGGCAAAGCCGAACGCATTAACATCACAATGCCCGCTTATTTAATTAAACGCAT
TGATGATTTTGTTGCTACCCATCCGCACTACAAAAGCCGTAGTCACTTTCTTGCCTCGGTTAGTGCAGATAAAATAATGG
CTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References