Detailed information of TA system TA196553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4113804..4114162
• Replicon: chromosome
• Accession: NZ_CP071522
• Organism: Escherichia coli strain UPEC129

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: J3P56_RS20145
• Protein ID: WP_001332330.1
• Coordinates: 4113804..4113926 (-)
• Length: 41 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: J3P56_RS20150
• Protein ID: WP_000125566.1
• Coordinates: 4113929..4114162 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3P56_RS20115	4109286..4110572	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
J3P56_RS20120	4110572..4111561	+	990	WP_000241229.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
J3P56_RS20125	4111558..4112379	+	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
J3P56_RS20130	4112382..4112759	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
J3P56_RS20135	4112766..4113614	+	849	WP_000257201.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
J3P56_RS20140	4113611..4113763	-	153	WP_146692624.1	CcdB family protein	-
J3P56_RS20145	4113804..4113926	-	123	WP_001332330.1	CcdB family protein	Toxin
J3P56_RS20150	4113929..4114162	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
J3P56_RS20155	4114248..4114727	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
J3P56_RS20160	4114919..4116781	-	1863	WP_000377122.1	glutathione-regulated potassium-efflux system protein KefC	-
J3P56_RS20165	4116774..4117304	-	531	WP_000600749.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
J3P56_RS20170	4117412..4118743	-	1332	WP_001183207.1	MFS transporter	-
J3P56_RS20175	4118802..4119089	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-39)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4563.34 Da
• Isoelectric Point: 10.9266

>T196553 WP_001332330.1 NZ_CP071522:c4113926-4113804 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPI

Nucleotide

• Length: 123 bp

>T196553 NZ_CP095156:152235-152651 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT196553 WP_000125566.1 NZ_CP071522:c4114162-4113929 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT196553 NZ_CP095156:152008-152238 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9