Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3684858..3685476 Replicon chromosome
Accession NZ_CP071522
Organism Escherichia coli strain UPEC129

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J3P56_RS18140 Protein ID WP_001291435.1
Coordinates 3685258..3685476 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J3P56_RS18135 Protein ID WP_000344800.1
Coordinates 3684858..3685232 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3P56_RS18125 3679948..3681141 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J3P56_RS18130 3681164..3684313 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
J3P56_RS18135 3684858..3685232 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J3P56_RS18140 3685258..3685476 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
J3P56_RS18145 3685650..3686201 + 552 WP_000102543.1 maltose O-acetyltransferase -
J3P56_RS18150 3686317..3686787 + 471 WP_000136192.1 YlaC family protein -
J3P56_RS18155 3686951..3688501 + 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J3P56_RS18160 3688543..3688896 - 354 WP_000878145.1 DUF1428 family protein -
J3P56_RS18170 3689275..3689586 + 312 WP_000409908.1 MGMT family protein -
J3P56_RS18175 3689617..3690189 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T196551 WP_001291435.1 NZ_CP071522:3685258-3685476 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T196551 NZ_CP095156:c86948-86799 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT196551 WP_000344800.1 NZ_CP071522:3684858-3685232 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT196551 NZ_CP095156:86992-87053 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References