Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4221729..4222226 Replicon chromosome
Accession NZ_CP071521
Organism Escherichia coli strain 11202

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag J2D70_RS20665 Protein ID WP_001173343.1
Coordinates 4221729..4221902 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag J2D70_RS20670 Protein ID WP_000398619.1
Coordinates 4221930..4222226 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J2D70_RS20655 (4218399) 4218399..4219856 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
J2D70_RS20660 (4220093) 4220093..4221610 + 1518 WP_001295074.1 lysine--tRNA ligase -
J2D70_RS20665 (4221729) 4221729..4221902 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
J2D70_RS20670 (4221930) 4221930..4222226 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
J2D70_RS20675 (4222453) 4222453..4222725 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
J2D70_RS20680 (4222737) 4222737..4222967 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
J2D70_RS20685 (4223148) 4223148..4224779 + 1632 WP_001216477.1 sensor histidine kinase -
J2D70_RS20690 (4224776) 4224776..4225495 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T196535 WP_001173343.1 NZ_CP071521:c4221902-4221729 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T196535 NZ_CP095155:1856483-1856734 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT196535 WP_000398619.1 NZ_CP071521:c4222226-4221930 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT196535 NZ_CP095155:1856731-1856985 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References