Detailed information of TA system TA196533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4040757..4041571
• Replicon: chromosome
• Accession: NZ_CP071521
• Organism: Escherichia coli strain 11202

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: J2D70_RS19745
• Protein ID: WP_001054376.1
• Coordinates: 4040757..4041014 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: J2D70_RS19750
• Protein ID: WP_001309181.1
• Coordinates: 4041026..4041571 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2D70_RS19725 (4036045)	4036045..4037151	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
J2D70_RS19730 (4037216)	4037216..4038196	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
J2D70_RS22905 (4038306)	4038306..4038511	+	206	Protein_3883	HNH endonuclease	-
J2D70_RS19735 (4038779)	4038779..4040019	-	1241	Protein_3884	helicase YjhR	-
J2D70_RS22910 (4040135)	4040135..4040266	+	132	WP_001309182.1	hypothetical protein	-
J2D70_RS19745 (4040757)	4040757..4041014	+	258	WP_001054376.1	YjhX family toxin	Toxin
J2D70_RS19750 (4041026)	4041026..4041571	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
J2D70_RS19755 (4041627)	4041627..4042373	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
J2D70_RS19760 (4042542)	4042542..4042760	+	219	Protein_3889	hypothetical protein	-
J2D70_RS19765 (4042798)	4042798..4042914	+	117	Protein_3890	VOC family protein	-
J2D70_RS19770 (4043159)	4043159..4044280	+	1122	WP_000010829.1	M42 family metallopeptidase	-
J2D70_RS19775 (4044277)	4044277..4044555	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
J2D70_RS19780 (4044567)	4044567..4045880	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4029344..4049795	20451

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T196533 WP_001054376.1 NZ_CP071521:4040757-4041014 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T196533 NZ_CP095155:1030770-1031105 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT196533 WP_001309181.1 NZ_CP071521:4041026-4041571 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT196533 NZ_CP095155:1030522-1030770 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8