Detailed information of TA system TA196527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3452028..3452646
• Replicon: chromosome
• Accession: NZ_CP071521
• Organism: Escherichia coli strain 11202

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: J2D70_RS16900
• Protein ID: WP_001290581.1
• Coordinates: 3452428..3452646 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: J2D70_RS16895
• Protein ID: WP_000344800.1
• Coordinates: 3452028..3452402 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2D70_RS16885 (3447117)	3447117..3448310	+	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
J2D70_RS16890 (3448333)	3448333..3451482	+	3150	WP_001132469.1	efflux RND transporter permease AcrB	-
J2D70_RS16895 (3452028)	3452028..3452402	+	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
J2D70_RS16900 (3452428)	3452428..3452646	+	219	WP_001290581.1	HHA domain-containing protein	Toxin
J2D70_RS16905 (3452818)	3452818..3453369	+	552	WP_000102564.1	maltose O-acetyltransferase	-
J2D70_RS16910 (3453485)	3453485..3453955	+	471	WP_000136192.1	YlaC family protein	-
J2D70_RS16915 (3454119)	3454119..3455669	+	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
J2D70_RS16920 (3455711)	3455711..3456064	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
J2D70_RS16930 (3456443)	3456443..3456754	+	312	WP_000409911.1	MGMT family protein	-
J2D70_RS16935 (3456785)	3456785..3457357	-	573	WP_000779842.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T196527 WP_001290581.1 NZ_CP071521:3452428-3452646 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T196527 NZ_CP095151:53921-54403 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT196527 WP_000344800.1 NZ_CP071521:3452028-3452402 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT196527 NZ_CP095151:53667-53933 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5