Detailed information of TA system TA196526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2375566..2376204
• Replicon: chromosome
• Accession: NZ_CP071521
• Organism: Escherichia coli strain 11202

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: J2D70_RS11505
• Protein ID: WP_000813794.1
• Coordinates: 2376028..2376204 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J2D70_RS11500
• Protein ID: WP_001270286.1
• Coordinates: 2375566..2375982 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2D70_RS11480 (2370718)	2370718..2371659	-	942	WP_001251304.1	ABC transporter permease	-
J2D70_RS11485 (2371660)	2371660..2372673	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
J2D70_RS11490 (2372691)	2372691..2373836	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
J2D70_RS11495 (2374081)	2374081..2375487	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
J2D70_RS11500 (2375566)	2375566..2375982	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
J2D70_RS11505 (2376028)	2376028..2376204	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
J2D70_RS11510 (2376426)	2376426..2376656	+	231	WP_000494244.1	YncJ family protein	-
J2D70_RS11515 (2376748)	2376748..2378709	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
J2D70_RS11520 (2378782)	2378782..2379318	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
J2D70_RS11525 (2379410)	2379410..2380585	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T196526 WP_000813794.1 NZ_CP071521:c2376204-2376028 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T196526 NZ_CP095150:c5345532-5345149 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT196526 WP_001270286.1 NZ_CP071521:c2375982-2375566 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT196526 NZ_CP095150:c5345774-5345532 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• PDB: 6HPB