Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1808323..1809154 Replicon chromosome
Accession NZ_CP071521
Organism Escherichia coli strain 11202

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag J2D70_RS08610 Protein ID WP_000854814.1
Coordinates 1808323..1808697 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag J2D70_RS08615 Protein ID WP_001285584.1
Coordinates 1808786..1809154 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J2D70_RS08570 (1803719) 1803719..1804885 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
J2D70_RS08575 (1805004) 1805004..1805477 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
J2D70_RS08580 (1805675) 1805675..1806733 + 1059 WP_001200891.1 FUSC family protein -
J2D70_RS08585 (1806905) 1806905..1807234 + 330 WP_000450409.1 DUF496 family protein -
J2D70_RS08590 (1807335) 1807335..1807469 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
J2D70_RS08595 (1807589) 1807589..1807717 + 129 Protein_1691 transposase domain-containing protein -
J2D70_RS08600 (1808006) 1808006..1808086 - 81 Protein_1692 hypothetical protein -
J2D70_RS08605 (1808132) 1808132..1808326 - 195 WP_000988600.1 DUF5983 family protein -
J2D70_RS08610 (1808323) 1808323..1808697 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
J2D70_RS08615 (1808786) 1808786..1809154 - 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
J2D70_RS08620 (1809228) 1809228..1809449 - 222 WP_000692323.1 DUF987 domain-containing protein -
J2D70_RS08625 (1809512) 1809512..1809958 - 447 WP_000187523.1 RadC family protein -
J2D70_RS08630 (1809955) 1809955..1811487 - 1533 WP_001350525.1 protein YeeR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T196515 WP_000854814.1 NZ_CP071521:c1808697-1808323 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T196515 NZ_CP095147:40180-40485 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT196515 WP_001285584.1 NZ_CP071521:c1809154-1808786 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT196515 NZ_CP095147:39960-40178 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGATATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References