Detailed information of TA system TA196513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 1117719..1119156
• Replicon: chromosome
• Accession: NZ_CP071521
• Organism: Escherichia coli strain 11202

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: J2D70_RS05395
• Protein ID: WP_000155570.1
• Coordinates: 1118083..1119156 (-)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: J2D70_RS05390
• Protein ID: WP_000461704.1
• Coordinates: 1117719..1118090 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2D70_RS22815 (1112920)	1112920..1113072	+	153	Protein_1053	arsenate reductase	-
J2D70_RS05360 (1113459)	1113459..1113926	-	468	WP_000702495.1	protein YfjT	-
J2D70_RS05365 (1113950)	1113950..1114393	-	444	WP_000824222.1	lipoprotein YfjS	-
J2D70_RS05370 (1114393)	1114393..1114629	-	237	WP_001144029.1	protein YpjK	-
J2D70_RS05375 (1114670)	1114670..1115371	-	702	WP_000189409.1	WYL domain-containing protein	-
J2D70_RS05380 (1115588)	1115588..1116409	-	822	WP_000197391.1	DUF932 domain-containing protein	-
J2D70_RS05385 (1116501)	1116501..1117364	-	864	WP_000787597.1	GTPase family protein	-
J2D70_RS05390 (1117719)	1117719..1118090	-	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
J2D70_RS05395 (1118083)	1118083..1119156	-	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
J2D70_RS05400 (1119298)	1119298..1119561	+	264	WP_000502050.1	DUF1524 domain-containing protein	-
J2D70_RS05405 (1119921)	1119921..1121537	+	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
J2D70_RS05410 (1121534)	1121534..1123723	+	2190	WP_000136402.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	luxS	1054296..1128915	74619

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T196513 WP_000155570.1 NZ_CP071521:c1119156-1118083 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T196513 NZ_CP095147:c5375-5070 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGGCGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAGCTCTATCCGGTGG
TGCACATCGGGGACGAGAGCTGGCGCCTGATGACCACGGACATGGCCAGCGTCCCGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCCGCCACCGGGAAAATGACATCAAAAACGCAATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT196513 WP_000461704.1 NZ_CP071521:c1118090-1117719 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT196513 NZ_CP095147:c5595-5377 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGATATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• PDB: 6Y2P
• AlphaFold DB: A0A373F4G4