Detailed information of TA system TA196500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 806449..806981
• Replicon: chromosome
• Accession: NZ_CP071508
• Organism: Staphylococcus haemolyticus strain 7b

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q4L7V3
• Locus tag: J2N84_RS04010
• Protein ID: WP_011275272.1
• Coordinates: 806616..806981 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: J2N84_RS04005
• Protein ID: WP_049426542.1
• Coordinates: 806449..806619 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2N84_RS03980	802235..802714	+	480	WP_053020220.1	PH domain-containing protein	-
J2N84_RS03985	802707..804230	+	1524	WP_053025516.1	PH domain-containing protein	-
J2N84_RS03990	804223..804750	+	528	WP_053025515.1	PH domain-containing protein	-
J2N84_RS03995	804760..805119	+	360	WP_049426544.1	holo-ACP synthase	-
J2N84_RS04000	805215..806363	+	1149	WP_053025513.1	alanine racemase	-
J2N84_RS04005	806449..806619	+	171	WP_049426542.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
J2N84_RS04010	806616..806981	+	366	WP_011275272.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J2N84_RS04015	807289..808290	+	1002	WP_053020219.1	PP2C family protein-serine/threonine phosphatase	-
J2N84_RS04020	808389..808715	+	327	WP_049426540.1	anti-sigma factor antagonist	-
J2N84_RS04025	808723..809196	+	474	WP_176743968.1	anti-sigma B factor RsbW	-
J2N84_RS04030	809171..809941	+	771	WP_011275276.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13672.75 Da
• Isoelectric Point: 8.4121

>T196500 WP_011275272.1 NZ_CP071508:806616-806981 [Staphylococcus haemolyticus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYRLDKDSVILLEQIR
TLDKKRLKEKLTYLSDEKMQEVDEALDISLGLHDEVKTQNT

Nucleotide

• Length: 366 bp

>T196500 NZ_CP095141:53921-54403 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6396.08 Da
• Isoelectric Point: 4.0325

>AT196500 WP_049426542.1 NZ_CP071508:806449-806619 [Staphylococcus haemolyticus]
MLTFNQNRSHSLEQSLKEGYSQMAELNLSLATEAFPLECEACDCNETYIISNYKSE

Nucleotide

• Length: 171 bp

>AT196500 NZ_CP095141:53667-53933 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A2A1KA75