Detailed information of TA system TA196497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 806449..806981
• Replicon: chromosome
• Accession: NZ_CP071505
• Organism: Staphylococcus haemolyticus strain 12b

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q4L7V3
• Locus tag: J2N82_RS04000
• Protein ID: WP_011275272.1
• Coordinates: 806616..806981 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: J2N82_RS03995
• Protein ID: WP_049426542.1
• Coordinates: 806449..806619 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2N82_RS03970	802235..802714	+	480	WP_053020220.1	PH domain-containing protein	-
J2N82_RS03975	802707..804230	+	1524	WP_053025516.1	PH domain-containing protein	-
J2N82_RS03980	804223..804750	+	528	WP_053025515.1	PH domain-containing protein	-
J2N82_RS03985	804760..805119	+	360	WP_049426544.1	holo-ACP synthase	-
J2N82_RS03990	805215..806363	+	1149	WP_053025513.1	alanine racemase	-
J2N82_RS03995	806449..806619	+	171	WP_049426542.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
J2N82_RS04000	806616..806981	+	366	WP_011275272.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J2N82_RS04005	807289..808290	+	1002	WP_053020219.1	PP2C family protein-serine/threonine phosphatase	-
J2N82_RS04010	808389..808715	+	327	WP_049426540.1	anti-sigma factor antagonist	-
J2N82_RS04015	808723..809196	+	474	WP_176743968.1	anti-sigma B factor RsbW	-
J2N82_RS04020	809171..809941	+	771	WP_011275276.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13672.75 Da
• Isoelectric Point: 8.4121

>T196497 WP_011275272.1 NZ_CP071505:806616-806981 [Staphylococcus haemolyticus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYRLDKDSVILLEQIR
TLDKKRLKEKLTYLSDEKMQEVDEALDISLGLHDEVKTQNT

Nucleotide

• Length: 366 bp

>T196497 NZ_CP095140:c4799407-4799123 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6396.08 Da
• Isoelectric Point: 4.0325

>AT196497 WP_049426542.1 NZ_CP071505:806449-806619 [Staphylococcus haemolyticus]
MLTFNQNRSHSLEQSLKEGYSQMAELNLSLATEAFPLECEACDCNETYIISNYKSE

Nucleotide

• Length: 171 bp

>AT196497 NZ_CP095140:c4799639-4799397 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A2A1KA75