Detailed information of TA system TA196495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 4129065..4130505
• Replicon: chromosome
• Accession: NZ_CP071504
• Organism: Shewanella sp. FJAT-53726

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: JYB88_RS18345
• Protein ID: WP_207325066.1
• Coordinates: 4129065..4130138 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: -
• Locus tag: JYB88_RS18350
• Protein ID: WP_207325067.1
• Coordinates: 4130140..4130505 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYB88_RS18315	4124140..4124532	+	393	WP_207325061.1	hypothetical protein	-
JYB88_RS18320	4124662..4125276	+	615	WP_207325062.1	recombinase family protein	-
JYB88_RS18325	4125393..4126073	-	681	WP_207325063.1	hypothetical protein	-
JYB88_RS18330	4126605..4126802	+	198	WP_207325064.1	helix-turn-helix domain-containing protein	-
JYB88_RS18335	4126817..4128456	+	1640	Protein_3536	DUF927 domain-containing protein	-
JYB88_RS18340	4128516..4128860	+	345	WP_207325065.1	excisionase family DNA-binding protein	-
JYB88_RS18345	4129065..4130138	+	1074	WP_207325066.1	type II toxin-antitoxin system RnlA family toxin	Toxin
JYB88_RS18350	4130140..4130505	+	366	WP_207325067.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
JYB88_RS18355	4130502..4131671	-	1170	WP_207325068.1	phage integrase SAM-like domain-containing protein	-
JYB88_RS18360	4131835..4133196	-	1362	WP_207325069.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
JYB88_RS18365	4133272..4134897	-	1626	WP_207325070.1	membrane protein insertase YidC	-
JYB88_RS18370	4134901..4135155	-	255	WP_207321444.1	membrane protein insertion efficiency factor YidD	-
JYB88_RS18375	4135122..4135478	-	357	WP_207321445.1	ribonuclease P protein component	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, DBD domain (203-325)

Antitoxin

• Antitoxin RnlB/LsoB (15-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 39999.76 Da
• Isoelectric Point: 6.0020

>T196495 WP_207325066.1 NZ_CP071504:4129065-4130138 [Shewanella sp. FJAT-53726]
MAERNYKNLNLDRAAIDGLVDNFAAQHQFTVESIGNTPGSQNGKRVVFGRIGTPYATVNVFFIKDGTSTIQFLTGLNPEL
GKQLADYLYDSINPAEFEQVNMVLEGFIEAAISPVLDLTAEESHIQVVVHSHDANRIVWKIQSKEYQDELTVTLHIGTRR
LQIQGRPLSCYRVFIFNLSELLDLQGLEKVLIRQEDGKAEIVQQEVARSYLRSVLGESYDQLHSNVEKLLISGLCVKLAS
PNLPDYCMLLYPELRSIEGALKSKLKAFGIVVGKDGFGEFFVKDQGVHILNADCAASCPNAEAAKVISEAYTFYNRERHG
LFHMEVLVEASRVISNITHLMTKSTQAWDLIKKLYTI

Nucleotide

• Length: 1074 bp

>T196495 NZ_CP095140:4120015-4120233 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13753.68 Da
• Isoelectric Point: 4.2564

>AT196495 WP_207325067.1 NZ_CP071504:4130140-4130505 [Shewanella sp. FJAT-53726]
MFNIRRLDLGLNSIAIVTAIGYENPLSSLSEIEHELTCLFGQELNGEVIFDLVCSNGLEWNRFMSLQIKNGFFDYNSVSI
VDKTDLLHQLIDKQAFFLSNNLHYLQESVLTSDEIPRVIGV

Nucleotide

• Length: 366 bp

>AT196495 NZ_CP095140:4119614-4119988 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG