Detailed information of TA system TA196489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1367016..1367793
• Replicon: chromosome
• Accession: NZ_CP071491
• Organism: Glaesserella parasuis strain GHP1807

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: J1G54_RS06855
• Protein ID: WP_207342635.1
• Coordinates: 1367293..1367793 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: U4SPD7
• Locus tag: J1G54_RS06850
• Protein ID: WP_005710653.1
• Coordinates: 1367016..1367303 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1G54_RS06830	1363384..1363884	-	501	WP_021115009.1	CvpA family protein	-
J1G54_RS06835	1363988..1365565	-	1578	WP_207342634.1	FAD-binding protein	-
J1G54_RS06840	1365740..1366477	-	738	WP_016527796.1	peptidoglycan editing factor PgeF	-
J1G54_RS06845	1366481..1366870	-	390	WP_021116694.1	RidA family protein	-
J1G54_RS06850	1367016..1367303	+	288	WP_005710653.1	DUF1778 domain-containing protein	Antitoxin
J1G54_RS06855	1367293..1367793	+	501	WP_207342635.1	GNAT family N-acetyltransferase	Toxin
J1G54_RS06860	1367821..1368822	+	1002	WP_010786647.1	nucleoid-associated protein YejK	-
J1G54_RS06865	1368883..1369218	+	336	WP_016527793.1	outer membrane protein assembly factor BamE	-
J1G54_RS06870	1369372..1370487	+	1116	WP_016527792.1	anhydro-N-acetylmuramic acid kinase	-
J1G54_RS06875	1370499..1371413	+	915	WP_021116535.1	N-acetylmuramic acid 6-phosphate etherase	-
J1G54_RS06880	1371455..1371769	+	315	WP_005710664.1	YqcC family protein	-
J1G54_RS06885	1371766..1372494	+	729	WP_021116534.1	tRNA pseudouridine(65) synthase TruC	-
J1G54_RS06890	1372512..1372667	+	156	WP_021116533.1	YoaH family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (4-87)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 19079.23 Da
• Isoelectric Point: 9.2835

>T196489 WP_207342635.1 NZ_CP071491:1367293-1367793 [Glaesserella parasuis]
MKSKFIEEPLTKHHQREAFDCGNLEINRFFKQYSRQSHEKGTSKTYISRHLDSQQIIGFYTITLSALDQQHLPELCKKRF
GYYPIPLFTLARLAVDIRYQKQGIGGMLLVKALKRCAIVAEQVGGIGLLIEAKDEDISRWYQSYGAIPLENMPLTLILLF
DTIKGL

Nucleotide

• Length: 501 bp

>T196489 NZ_CP095138:c128956-128651 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10917.47 Da
• Isoelectric Point: 6.4650

>AT196489 WP_005710653.1 NZ_CP071491:1367016-1367303 [Glaesserella parasuis]
MPQIANALNDRMSIRISTEDKQTLIRAVALTHTNITEFVLQRILPSAREIIQQYDNRQFSPQDLSLIMELLDNPPKANTK
LQQAAKLAKESYHEE

Nucleotide

• Length: 288 bp

>AT196489 NZ_CP095138:c129176-128958 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: U4SPD7