Detailed information of TA system TA196485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 639402..639982
• Replicon: chromosome
• Accession: NZ_CP071491
• Organism: Glaesserella parasuis strain GHP1807

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A143CFE3
• Locus tag: J1G54_RS03500
• Protein ID: WP_005710599.1
• Coordinates: 639402..639746 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: J1G54_RS03505
• Protein ID: WP_207342833.1
• Coordinates: 639743..639982 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1G54_RS03490	637324..638715	-	1392	WP_207342832.1	MATE family efflux transporter	-
J1G54_RS03495	638728..639396	-	669	WP_005710597.1	bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA	-
J1G54_RS03500	639402..639746	-	345	WP_005710599.1	endoribonuclease MazF	Toxin
J1G54_RS03505	639743..639982	-	240	WP_207342833.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J1G54_RS03510	640168..641043	+	876	WP_021119465.1	putative selenate ABC transporter substrate-binding protein	-
J1G54_RS03515	641155..641904	+	750	WP_005710605.1	phosphonate ABC transporter ATP-binding protein	-
J1G54_RS03520	641894..642679	+	786	WP_016528266.1	ABC transporter permease subunit	-
J1G54_RS03525	642676..643524	+	849	WP_075592852.1	ABC transporter permease subunit	-
J1G54_RS03530	643595..644287	+	693	WP_021114597.1	tellurite resistance TerB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12830.81 Da
• Isoelectric Point: 7.9277

>T196485 WP_005710599.1 NZ_CP071491:c639746-639402 [Glaesserella parasuis]
MTTQEYIPDVGDIIWLDFDPQAGHEQAGHRPALVLTPTIYNRQTGLLICCPLTTKVKGYPFEVNIAGTPQNVVLSDQIKS
LDWRIRHAKFKGKINHHQLSEVKAKIATLLQISE

Nucleotide

• Length: 345 bp

>T196485 NZ_CP095137:c4145670-4145383 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8796.24 Da
• Isoelectric Point: 5.0351

>AT196485 WP_207342833.1 NZ_CP071491:c639982-639743 [Glaesserella parasuis]
MQVAIKKWGNSVGIRIPALLSNSLKLQAESLVDIQEVDGRLIIEPIKPKYNLEQLLSGITEDNLHNEVDFGKPVGKEML

Nucleotide

• Length: 240 bp

>AT196485 NZ_CP095137:c4145396-4145094 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A143CFE3