Detailed information of TA system TA196482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 287931..289618
• Replicon: chromosome
• Accession: NZ_CP071491
• Organism: Glaesserella parasuis strain GHP1807

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U4SB19
• Locus tag: J1G54_RS01630
• Protein ID: WP_021111224.1
• Coordinates: 287931..289274 (+)
• Length: 448 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J1G54_RS01635
• Protein ID: WP_015940035.1
• Coordinates: 289274..289618 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1G54_RS01605	283901..284524	-	624	WP_160404946.1	TonB-dependent receptor	-
J1G54_RS01610	284753..284977	+	225	Protein_311	hypothetical protein	-
J1G54_RS01615	285220..286899	+	1680	WP_005713398.1	DNA repair protein RecN	-
J1G54_RS01620	286979..287206	-	228	Protein_313	transposase	-
J1G54_RS01625	287380..287817	+	438	WP_005713142.1	ribosomal protein S18-alanine N-acetyltransferase	-
J1G54_RS01630	287931..289274	+	1344	WP_021111224.1	type II toxin-antitoxin system HipA family toxin	Toxin
J1G54_RS01635	289274..289618	+	345	WP_015940035.1	helix-turn-helix domain-containing protein	Antitoxin
J1G54_RS01640	289647..290972	+	1326	WP_021111225.1	ATP-dependent RNA helicase SrmB	-
J1G54_RS01645	291093..291725	+	633	WP_021110345.1	lipoprotein insertase outer membrane protein LolB	-
J1G54_RS01650	291727..292572	+	846	WP_075606354.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
J1G54_RS01655	292600..293550	+	951	WP_005713153.1	ribose-phosphate pyrophosphokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (161-407)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 448 a.a.
• Molecular weight: 51106.20 Da
• Isoelectric Point: 5.4867

>T196482 WP_021111224.1 NZ_CP071491:287931-289274 [Glaesserella parasuis]
MQIEPLILQAYIADRWQDIAILTFQADYSLNDFSYLHDLTEILSDNDKAVSFNYPVEFFPHRSVSGWFAFLDDIVPSGAS
RRYWLQKLDIAHLPMAQQNYLLLKQGTIAPVGHLRVKQAVSSFVENEPSRLFEIDDVINRQADFLEYANQRGAMAGGATG
AGGEAPKLLLRQTATRQVWIDNLQQGASSDDFYLVKFPRGKRSVIDCDILRAEFHYYHELASMGFDTIDTTKMRLEEGER
YPSLWLPRFDVYRNEQGKMARYAVESVYAMLEKGAGSLLDHEKVIRQLIAKIETSHLVETGMFFDKQAFVIEWVRRDLLN
IAFGNSDNHGRNTAFLRNQNRIWLAPIYDFAPMKADPEGIPRTTMWDSKGHPALELGGEYRFDLIAEQLDDLCPADKLLD
ALRQTAQQLVGLKARLEARGVPTAILTYPSISLDYLPEKLKRWQLLS

Nucleotide

• Length: 1344 bp

>T196482 NZ_CP095137:3622961-3623179 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13225.52 Da
• Isoelectric Point: 10.2782

>AT196482 WP_015940035.1 NZ_CP071491:289274-289618 [Glaesserella parasuis]
MEEIKQARASKSKKTLDIYQRATVRDEIARKLLLNEMSLGQALKYLRLHLLAMKQERYAEIVKVSRKTLSDLENDKGNYS
IDIINQVLRPFELQLGVVPMNKTLLRQVLNEQAV

Nucleotide

• Length: 345 bp

>AT196482 NZ_CP095137:3622561-3622935 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: U4SB19