Detailed information of TA system TA196477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1261854..1262504
• Replicon: chromosome
• Accession: NZ_CP071490
• Organism: Glaesserella parasuis strain GHP1808

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7H8UVF2
• Locus tag: J1G53_RS05945
• Protein ID: WP_016527644.1
• Coordinates: 1261854..1262030 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A836YYB9
• Locus tag: J1G53_RS05950
• Protein ID: WP_016527643.1
• Coordinates: 1262088..1262504 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1G53_RS05925	1257512..1257841	-	330	WP_016527647.1	phage tail protein	-
J1G53_RS05930	1257846..1260296	-	2451	WP_038513529.1	phage tail length tape measure family protein	-
J1G53_RS05935	1260522..1260980	-	459	WP_016527646.1	hypothetical protein	-
J1G53_RS05940	1261085..1261756	-	672	WP_016527645.1	hypothetical protein	-
J1G53_RS05945	1261854..1262030	+	177	WP_016527644.1	type II toxin-antitoxin system HicA family toxin	Toxin
J1G53_RS05950	1262088..1262504	+	417	WP_016527643.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J1G53_RS05955	1262550..1263029	-	480	WP_016527642.1	hypothetical protein	-
J1G53_RS05960	1263038..1263412	-	375	WP_016527641.1	hypothetical protein	-
J1G53_RS05965	1263409..1263777	-	369	WP_035490850.1	hypothetical protein	-
J1G53_RS05970	1263777..1264124	-	348	WP_035490848.1	hypothetical protein	-
J1G53_RS05975	1264125..1264502	-	378	WP_016527638.1	hypothetical protein	-
J1G53_RS05980	1264505..1264756	-	252	WP_207342402.1	hypothetical protein	-
J1G53_RS05985	1264768..1265757	-	990	WP_016527636.1	encapsulin	-
J1G53_RS05990	1265771..1266205	-	435	WP_035490842.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1249433..1337499	88066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-60)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.74 Da
• Isoelectric Point: 10.6914

>T196477 WP_016527644.1 NZ_CP071490:1261854-1262030 [Glaesserella parasuis]
VKQSEFLRWLKANGVEVENGTKHLKLYYNGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T196477 NZ_CP095137:c1984414-1984040 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTACTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15470.82 Da
• Isoelectric Point: 4.5304

>AT196477 WP_016527643.1 NZ_CP071490:1262088-1262504 [Glaesserella parasuis]
MFYPALFTPAEEGGFVVTFPDIPEAITQGDTFEEAMEMAEDVLLSSVEIYFDDERAFPLSRPAGIYETSVFIPESVYAKI
LLHNTMCEKFISKAEVARLNNIKPPEIHRILAPRHTTRIDTIGRILASLGRPLQLSLA

Nucleotide

• Length: 417 bp

>AT196477 NZ_CP095137:c1984871-1984503 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCAGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A7H8UVF2

Antitoxin

• AlphaFold DB: A0A836YYB9