Detailed information of TA system TA196474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 378280..379967
• Replicon: chromosome
• Accession: NZ_CP071490
• Organism: Glaesserella parasuis strain GHP1808

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: J1G53_RS01855
• Protein ID: WP_160416784.1
• Coordinates: 378280..379623 (+)
• Length: 448 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J1G53_RS01860
• Protein ID: WP_005713145.1
• Coordinates: 379623..379967 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1G53_RS01830	373281..375458	-	2178	WP_075592552.1	bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL	-
J1G53_RS01835	375608..376549	-	942	WP_075592553.1	16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC	-
J1G53_RS01840	376632..377045	+	414	WP_015940031.1	DNA polymerase III subunit psi	-
J1G53_RS01845	377242..377487	+	246	WP_075605722.1	M23 family metallopeptidase	-
J1G53_RS01850	377729..378166	+	438	WP_160416782.1	ribosomal protein S18-alanine N-acetyltransferase	-
J1G53_RS01855	378280..379623	+	1344	WP_160416784.1	HipA domain-containing protein	Toxin
J1G53_RS01860	379623..379967	+	345	WP_005713145.1	helix-turn-helix domain-containing protein	Antitoxin
J1G53_RS01865	379996..381321	+	1326	WP_044009187.1	ATP-dependent RNA helicase SrmB	-
J1G53_RS01870	381439..382071	+	633	WP_159179811.1	lipoprotein insertase outer membrane protein LolB	-
J1G53_RS01875	382073..382918	+	846	WP_075605726.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
J1G53_RS01880	382946..383896	+	951	WP_005713153.1	ribose-phosphate pyrophosphokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (161-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 448 a.a.
• Molecular weight: 50974.89 Da
• Isoelectric Point: 5.1119

>T196474 WP_160416784.1 NZ_CP071490:378280-379623 [Glaesserella parasuis]
MQIEPLTLQAYIADRWQDIAILTFQADYSLNDFSYLYDLAEILSDNDKAVSFNYPVEFFPHRSVSGWFAFLDDIVPSGAS
RRYWLQKLDIAHLPMAQQNYLLLKQGTIAPVGHLRVKQAVSSFVENEPSRLFEIDDVINRQADFLEYANQRGAMAGGATG
AGGEAPKLLLRQTATQQVWIDNLQQGDAADDFYLVKFPRGKRSAIDCDILRAEFHYYHELASMGFDTIDTTKMRLEEGER
YPSLWLPRFDVYRNEQGKMARYAVESVYAMLEKGAGSLLDHEKVIRQLIAKIENSHLVETGMAFDKQAFVIEWVRRDLLN
IAFGNSDNHGRNTAFLRNQNRIWLAPIYDFAPMKADPEGIPRTTMWDSKSHPALELGGEYRFDLIAEQLDDLCSADKLLD
ALRQTAQQLAGLKARLEARGVPTAILTYPSISLDYLPEKLKRWQLLS

Nucleotide

• Length: 1344 bp

>T196474 NZ_CP095137:1020214-1020621 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13207.48 Da
• Isoelectric Point: 10.2782

>AT196474 WP_005713145.1 NZ_CP071490:379623-379967 [Glaesserella parasuis]
MEEIKQARASKSKKTLDIYQRATVRDEIARKLLLNEMSLGQALKYLRLHLLAMKQERYAEIVKVSRKTLSDLENDKGNYS
IDIINQVLRPFELQLGVVPINKTLLRQVLNEQAV

Nucleotide

• Length: 345 bp

>AT196474 NZ_CP095137:1019967-1020233 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA