Detailed information of TA system TA196458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5058581..5060262
• Replicon: chromosome
• Accession: NZ_CP071464
• Organism: Klebsiella pneumoniae strain C2244

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: W8V1Q6
• Locus tag: J1C69_RS25015
• Protein ID: WP_002885155.1
• Coordinates: 5058581..5059498 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: W8UPQ8
• Locus tag: J1C69_RS25020
• Protein ID: WP_004151732.1
• Coordinates: 5059504..5060262 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1C69_RS24995	5053887..5055407	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
J1C69_RS25000	5055404..5056402	+	999	WP_002885172.1	ribose ABC transporter permease	-
J1C69_RS25005	5056433..5057374	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
J1C69_RS25010	5057449..5058456	+	1008	WP_004151731.1	carbohydrate kinase family protein	-
J1C69_RS25015	5058581..5059498	-	918	WP_002885155.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
J1C69_RS25020	5059504..5060262	-	759	WP_004151732.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
J1C69_RS25025	5060348..5061076	-	729	WP_002885152.1	response regulator transcription factor	-
J1C69_RS25030	5061349..5063496	+	2148	WP_077598858.1	formate dehydrogenase subunit alpha	-
J1C69_RS25035	5063726..5064412	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34687.15 Da
• Isoelectric Point: 5.4707

>T196458 WP_002885155.1 NZ_CP071464:c5059498-5058581 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T196458 NZ_CP095132:c3312740-3312564 [Klebsiella pneumoniae]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGTATCAAATAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28564.92 Da
• Isoelectric Point: 10.0870

>AT196458 WP_004151732.1 NZ_CP071464:c5060262-5059504 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLSFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT196458 NZ_CP095132:c3312516-3312109 [Klebsiella pneumoniae]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
AGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCCTGGTAACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCTTGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGTACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GH11

Antitoxin

• AlphaFold DB: A0A0H3GQB5