Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4799754..4800564 Replicon chromosome
Accession NZ_CP071464
Organism Klebsiella pneumoniae strain C2244

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag J1C69_RS23745 Protein ID WP_002887280.1
Coordinates 4799754..4800287 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag J1C69_RS23750 Protein ID WP_002887278.1
Coordinates 4800298..4800564 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1C69_RS23740 4798585..4799706 + 1122 WP_002887282.1 cupin domain-containing protein -
J1C69_RS23745 4799754..4800287 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
J1C69_RS23750 4800298..4800564 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
J1C69_RS23755 4800667..4802100 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
J1C69_RS23760 4802090..4802773 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
J1C69_RS23765 4802945..4804330 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
J1C69_RS23770 4804348..4804692 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T196456 WP_002887280.1 NZ_CP071464:c4800287-4799754 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T196456 NZ_CP095132:1974430-1974532 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT196456 WP_002887278.1 NZ_CP071464:c4800564-4800298 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT196456 NZ_CP095132:c1974539-1974394 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References