Detailed information of TA system TA196439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 364083..364870
• Replicon: plasmid p2
• Accession: NZ_CP071456
• Organism: Rhizobium lentis strain BLR27

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: J0663_RS28010
• Protein ID: WP_207245687.1
• Coordinates: 364083..364577 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: J0663_RS28015
• Protein ID: WP_207245688.1
• Coordinates: 364574..364870 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0663_RS27990	359613..361550	-	1938	WP_207245683.1	hypothetical protein	-
J0663_RS27995	361547..362161	-	615	WP_207245684.1	DUF1768 domain-containing protein	-
J0663_RS28000	362342..362761	+	420	WP_207245685.1	hypothetical protein	-
J0663_RS28005	362937..363833	-	897	WP_207245686.1	diguanylate cyclase	-
J0663_RS28010	364083..364577	-	495	WP_207245687.1	GNAT family N-acetyltransferase	Toxin
J0663_RS28015	364574..364870	-	297	WP_207245688.1	DUF1778 domain-containing protein	Antitoxin
J0663_RS28020	364999..367074	-	2076	WP_207245689.1	carboxy terminal-processing peptidase	-
J0663_RS28025	367307..367606	+	300	WP_207245690.1	hypothetical protein	-
J0663_RS28030	367698..368897	-	1200	WP_207245691.1	mandelate racemase/muconate lactonizing enzyme family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..422827	422827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (28-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (16-90)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17792.40 Da
• Isoelectric Point: 8.0784

>T196439 WP_207245687.1 NZ_CP071456:c364577-364083 [Rhizobium lentis]
VTLSPPAPLADHHELVEFGSGVPELDDWLRRRARANQASGASRTFVVCEANRVVAYYALASGAVRQPEAPGRFRRNMPDP
IPVAVLGRLAIDRSYQGRGLGRALVRDAGLRLLNAAEIIGIRALLVHAISDEARAFYEAVGFLPSPSDPMMQMVGLHDLD
SALR

Nucleotide

• Length: 495 bp

>T196439 NZ_CP095125:2662166-2662273 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11013.62 Da
• Isoelectric Point: 8.5308

>AT196439 WP_207245688.1 NZ_CP071456:c364870-364574 [Rhizobium lentis]
MPTSTTNKSPDASVPLNMRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRAIVTVSPEIYAEYLARLDAPAK
PNERLKRTMSTKAPWDEA

Nucleotide

• Length: 297 bp

>AT196439 NZ_CP095125:c2662119-2662053 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT