Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 1981..2540 Replicon plasmid p2
Accession NZ_CP071443
Organism Escherichia coli strain EcPF20

Toxin (Protein)


Gene name relE Uniprot ID U9YB49
Locus tag J1F34_RS23920 Protein ID WP_000421259.1
Coordinates 2265..2540 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID U9Y625
Locus tag J1F34_RS23915 Protein ID WP_001178087.1
Coordinates 1981..2265 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F34_RS23910 46..1077 + 1032 WP_000907852.1 replication initiation protein -
J1F34_RS23915 1981..2265 + 285 WP_001178087.1 ribbon-helix-helix domain-containing protein Antitoxin
J1F34_RS23920 2265..2540 + 276 WP_000421259.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J1F34_RS23925 2646..2927 + 282 WP_001105066.1 hypothetical protein -
J1F34_RS23930 3119..3385 - 267 WP_000627832.1 hypothetical protein -
J1F34_RS23935 3385..3879 - 495 WP_077127980.1 hypothetical protein -
J1F34_RS23940 3935..4537 - 603 WP_061671620.1 proQ/FINO family protein -
J1F34_RS23945 4891..6237 + 1347 WP_001132027.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..88572 88572


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10736.45 Da        Isoelectric Point: 9.9324

>T196429 WP_000421259.1 NZ_CP071443:2265-2540 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T196429 NZ_CP095125:184380-184487 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10488.92 Da        Isoelectric Point: 5.6558

>AT196429 WP_001178087.1 NZ_CP071443:1981-2265 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT196429 NZ_CP095125:c184323-184266 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YB49

Antitoxin

Source ID Structure
AlphaFold DB U9Y625

References