Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 50967..51514 Replicon plasmid p1
Accession NZ_CP071442
Organism Escherichia coli strain EcPF20

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag J1F34_RS23290 Protein ID WP_000969996.1
Coordinates 50967..51248 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag J1F34_RS23295 Protein ID WP_000079941.1
Coordinates 51245..51514 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F34_RS23260 46397..46657 + 261 WP_000343091.1 hypothetical protein -
J1F34_RS23265 46654..47244 + 591 WP_151340509.1 hypothetical protein -
J1F34_RS23270 47264..47521 - 258 WP_000142431.1 hypothetical protein -
J1F34_RS23275 47650..47985 + 336 WP_001080729.1 colicin 1A immunity protein -
J1F34_RS23280 48007..49887 - 1881 WP_112039468.1 colicin -
J1F34_RS23285 50073..50921 - 849 WP_001057989.1 3'-5' exonuclease -
J1F34_RS23290 50967..51248 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J1F34_RS23295 51245..51514 - 270 WP_000079941.1 hypothetical protein Antitoxin
J1F34_RS23300 52158..53909 - 1752 Protein_46 Tn3-like element TnAs1 family transposase -
J1F34_RS23305 53898..54305 + 408 WP_001214976.1 cysteine hydrolase -
J1F34_RS23310 54443..55327 + 885 WP_000058717.1 EamA family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA5 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-Ia / sitABCD iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA 1..159570 159570


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T196428 WP_000969996.1 NZ_CP071442:c51248-50967 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T196428 NZ_CP095125:183897-184004 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT196428 WP_000079941.1 NZ_CP071442:c51514-51245 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT196428 NZ_CP095125:c183849-183783 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References