Detailed information of TA system TA196427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 39008..39384
• Replicon: plasmid p1
• Accession: NZ_CP071442
• Organism: Escherichia coli strain EcPF20

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: J1F34_RS23210
• Protein ID: WP_001514250.1
• Coordinates: 39008..39190 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: J1F34_RS23215
• Protein ID: WP_001324224.1
• Coordinates: 39187..39384 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F34_RS23200	37007..38041	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
J1F34_RS23205	38601..38909	-	309	WP_000377483.1	hypothetical protein	-
J1F34_RS23210	39008..39190	-	183	WP_001514250.1	hypothetical protein	Toxin
J1F34_RS23215	39187..39384	-	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
J1F34_RS23220	40066..41340	+	1275	WP_174412140.1	HlyD family secretion protein	-
J1F34_RS23225	41315..43429	+	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
J1F34_RS23230	43599..43910	-	312	WP_001259758.1	colicin V	-
J1F34_RS23235	43888..44124	-	237	WP_001323890.1	colicin V immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA5 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-Ia / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..159570	159570

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6961.10 Da
• Isoelectric Point: 4.5349

>T196427 WP_001514250.1 NZ_CP071442:c39190-39008 [Escherichia coli]
MMEIFWTMLTSQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T196427 NZ_CP095122:136971-137387 [Klebsiella pneumoniae]
GTGAACAAAATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATTACCTACGCCGAGATGCGCTTCGGCGCCACCGGGC
CGAAGGCCGCGCCGCGTCACGTCCAGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCATGGCCGGCACGCCGATTGGCCCGAACGACACGGCGATTGC
CGGACACGCCATCGCCGCCGGCGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTGCCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT196427 WP_001324224.1 NZ_CP071442:c39384-39187 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT196427 NZ_CP095122:136744-136974 [Klebsiella pneumoniae]
ATGCGCACAGTCTCTATTTTTAAAAACGGCAACAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA