196423

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3686234..3686810 Replicon chromosome
Accession NZ_CP071441
Organism Escherichia coli strain EcPF20

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag J1F34_RS17585 Protein ID WP_001297643.1
Coordinates 3686234..3686521 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag J1F34_RS17590 Protein ID WP_000063160.1
Coordinates 3686508..3686810 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F34_RS17570 3682471..3683751 - 1281 WP_001037390.1 DUF445 domain-containing protein -
J1F34_RS17575 3683792..3685024 - 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
J1F34_RS17580 3685339..3686035 + 697 Protein_3424 IS1 family transposase -
J1F34_RS17585 3686234..3686521 + 288 WP_001297643.1 BrnT family toxin Toxin
J1F34_RS17590 3686508..3686810 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
J1F34_RS17595 3686844..3687800 - 957 WP_001297640.1 GTPase -
J1F34_RS17600 3687811..3688014 - 204 WP_000467859.1 YbdD/YjiX family protein -
J1F34_RS17605 3688064..3690214 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3685826..3686035 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T196423 WP_001297643.1 NZ_CP071441:3686234-3686521 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T196423 NZ_CP095121:c4507833-4507300 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT196423 WP_000063160.1 NZ_CP071441:3686508-3686810 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT196423 NZ_CP095121:c4508110-4507844 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References