Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 3521125..3521720 Replicon chromosome
Accession NZ_CP071441
Organism Escherichia coli strain EcPF20

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag J1F34_RS16770 Protein ID WP_000239579.1
Coordinates 3521370..3521720 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag J1F34_RS16765 Protein ID WP_001223208.1
Coordinates 3521125..3521376 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F34_RS16755 3516789..3520568 + 3780 WP_000060901.1 autotransporter assembly complex protein TamB -
J1F34_RS16760 3520571..3520912 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
J1F34_RS16765 3521125..3521376 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
J1F34_RS16770 3521370..3521720 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
J1F34_RS16775 3521800..3522330 - 531 WP_000055072.1 inorganic diphosphatase -
J1F34_RS16780 3522640..3523596 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
J1F34_RS16785 3523906..3525408 + 1503 WP_021548597.1 sugar ABC transporter ATP-binding protein -
J1F34_RS16790 3525422..3526444 + 1023 WP_001339490.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T196420 WP_000239579.1 NZ_CP071441:3521370-3521720 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T196420 NZ_CP095121:1945792-1945894 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT196420 WP_001223208.1 NZ_CP071441:3521125-3521376 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT196420 NZ_CP095121:c1945901-1945756 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References