Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2057903..2058557 Replicon chromosome
Accession NZ_CP071441
Organism Escherichia coli strain EcPF20

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag J1F34_RS09800 Protein ID WP_000244772.1
Coordinates 2057903..2058310 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag J1F34_RS09805 Protein ID WP_000354046.1
Coordinates 2058291..2058557 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F34_RS09780 2053860..2055593 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
J1F34_RS09785 2055599..2056309 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J1F34_RS09790 2056334..2057230 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
J1F34_RS09795 2057342..2057863 + 522 WP_001055874.1 flavodoxin FldB -
J1F34_RS09800 2057903..2058310 - 408 WP_000244772.1 protein YgfX Toxin
J1F34_RS09805 2058291..2058557 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
J1F34_RS09810 2058800..2059780 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
J1F34_RS09815 2059857..2060516 - 660 WP_000250274.1 hemolysin III family protein -
J1F34_RS09820 2060680..2060991 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
J1F34_RS09825 2061036..2062469 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
J1F34_RS09830 2062526..2063269 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T196415 WP_000244772.1 NZ_CP071441:c2058310-2057903 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T196415 NZ_CP095119:c2531097-2530831 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAAAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT196415 WP_000354046.1 NZ_CP071441:c2058557-2058291 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT196415 NZ_CP095119:c2531348-2531097 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACCTCTACTGACGATAAAAATGTAGTAATCATGTCAGAATCAGACTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAACAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References