Detailed information of TA system TA196413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1619325..1619950
• Replicon: chromosome
• Accession: NZ_CP071441
• Organism: Escherichia coli strain EcPF20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J1F34_RS07680
• Protein ID: WP_000911330.1
• Coordinates: 1619325..1619723 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: J1F34_RS07685
• Protein ID: WP_000450524.1
• Coordinates: 1619723..1619950 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F34_RS07660	1615203..1615403	+	201	WP_000383836.1	YpfN family protein	-
J1F34_RS07665	1615513..1616211	-	699	WP_000679823.1	esterase	-
J1F34_RS07670	1616285..1618300	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
J1F34_RS07675	1618315..1619178	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
J1F34_RS07680	1619325..1619723	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
J1F34_RS07685	1619723..1619950	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
J1F34_RS07690	1620104..1620817	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
J1F34_RS07695	1621030..1622064	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
J1F34_RS07700	1622081..1622959	-	879	WP_113976360.1	4-hydroxy-tetrahydrodipicolinate synthase	-
J1F34_RS07705	1623105..1623677	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
J1F34_RS07710	1623677..1624147	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T196413 WP_000911330.1 NZ_CP071441:c1619723-1619325 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T196413 NZ_CP095119:c2218357-2218148 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACATCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAACATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTTTATTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT196413 WP_000450524.1 NZ_CP071441:c1619950-1619723 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT196413 NZ_CP095119:c2218662-2218369 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33