Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1133210..1134041 Replicon chromosome
Accession NZ_CP071441
Organism Escherichia coli strain EcPF20

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag J1F34_RS05480 Protein ID WP_000854814.1
Coordinates 1133667..1134041 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag J1F34_RS05475 Protein ID WP_001285585.1
Coordinates 1133210..1133578 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F34_RS05440 1128442..1129512 + 1071 WP_000102664.1 patatin-like phospholipase family protein -
J1F34_RS05445 1129648..1130331 + 684 WP_023147547.1 hypothetical protein -
J1F34_RS05450 1130347..1130757 + 411 WP_000846713.1 hypothetical protein -
J1F34_RS05455 1130978..1131799 + 822 WP_001234530.1 DUF945 domain-containing protein -
J1F34_RS05460 1131881..1132360 + 480 WP_000860076.1 antirestriction protein -
J1F34_RS05465 1132376..1132852 + 477 WP_001186773.1 RadC family protein -
J1F34_RS05470 1132915..1133136 + 222 WP_000692323.1 DUF987 domain-containing protein -
J1F34_RS05475 1133210..1133578 + 369 WP_001285585.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
J1F34_RS05480 1133667..1134041 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
J1F34_RS05485 1134038..1134232 + 195 WP_000988600.1 hypothetical protein -
J1F34_RS05490 1134245..1134358 + 114 WP_001161660.1 DUF957 domain-containing protein -
J1F34_RS05495 1134647..1134775 - 129 Protein_1082 transposase domain-containing protein -
J1F34_RS05500 1134847..1135029 + 183 WP_001016348.1 hypothetical protein -
J1F34_RS05505 1135130..1135459 - 330 WP_000450409.1 DUF496 family protein -
J1F34_RS05510 1135631..1136689 - 1059 WP_029364112.1 FUSC family protein -
J1F34_RS05515 1136887..1137360 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
J1F34_RS05520 1137479..1138645 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T196411 WP_000854814.1 NZ_CP071441:1133667-1134041 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T196411 NZ_CP095119:c2017704-2017597 [Staphylococcus aureus]
GTGGTATCTGTTGTTGATGCACTAAATTTAATGTTTAGTTTCGGTATGTTTATCGTTACTTTACTTGGTTTAGTCATCGC
AATCGTTAAATTAAACCATAAAAAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT196411 WP_001285585.1 NZ_CP071441:1133210-1133578 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT196411 NZ_CP095119:2017488-2017542 [Staphylococcus aureus]
AAAAAGGCAACATGCGTAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References